Differences in Gluco and Galacto Substrate-Binding Interactions in a Dual 6Pβ-Glucosidase/6Pβ-Galactosidase Glycoside Hydrolase 1 Enzyme from

Bacillus licheniformis / metabolism Galactosidases Glucosidases / metabolism Glycoside Hydrolases / metabolism Substrate Specificity

Journal

Journal of chemical information and modeling
ISSN: 1549-960X
Titre abrégé: J Chem Inf Model
Pays: United States
ID NLM: 101230060

Informations de publication

Date de publication:
27 09 2021
Historique:
pubmed: 24 8 2021
medline: 25 2 2023
entrez: 23 8 2021
Statut: ppublish

Résumé

Bacterial glycoside hydrolase 1 (GH1) enzymes with 6-phospho-β-galactosidase and 6-phospho-β-glucosidase activities have the important task of releasing phosphorylated and nonphosphorylated monosaccharides into the cytoplasm. Curiously, dual 6-phospho-β-galactosidase/6-phospho-β-glucosidase (dual-phospho) enzymes have broad specificity and are able to hydrolyze galacto- and gluco-derived substrates. This study investigates the structure and substrate specificity of a GH family 1 enzyme from

Identifiants

DOI: 10.1021/acs.jcim.1c00413 PMID: 34423980
pubmed: 34423980
doi: 10.1021/acs.jcim.1c00413
doi:

Substances chimiques

Galactosidases EC 3.2.1.-
Glucosidases EC 3.2.1.-
Glycoside Hydrolases EC 3.2.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4554-4570

Auteurs

Wayde Veldman (W)

Research Unit in Bioinformatics (RUBi), Department of Biochemistry and Microbiology, Rhodes University, Grahamstown 6140, South Africa.
ORCID: 0000-0002-2747-7146

Marcelo Vizona Liberato (MV)

São Carlos Institute of Physics, University of São Paulo, São Carlos 13566-590, Brazil.

Valquiria P Souza (VP)

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-000, Brazil.

Vitor M Almeida (VM)

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-000, Brazil.

Sandro R Marana (SR)

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-000, Brazil.

Özlem Tastan Bishop (Ö)

Research Unit in Bioinformatics (RUBi), Department of Biochemistry and Microbiology, Rhodes University, Grahamstown 6140, South Africa.
ORCID: 0000-0001-6861-7849

Igor Polikarpov (I)

São Carlos Institute of Physics, University of São Paulo, São Carlos 13566-590, Brazil.
ORCID: 0000-0001-9496-4174

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus licheniformis › Differences in Gluco and Galacto...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Galactosidases › Differences in Gluco and Galacto...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Glucosidases › Differences in Gluco and Galacto...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Differences in Gluco and Galacto...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Differences in Gluco and Galacto...