Specific Recognition of a Stem-Loop RNA Structure by the Alphavirus Capsid Protein.
Alphavirus
/ genetics
Binding Sites
Capsid
/ metabolism
Capsid Proteins
/ genetics
Cell Line
Chikungunya virus
/ genetics
Genome, Viral
/ genetics
Inverted Repeat Sequences
/ genetics
Protein Binding
RNA, Viral
/ genetics
RNA-Binding Motifs
Ross River virus
/ genetics
Semliki forest virus
/ genetics
Virus Assembly
RNA binding
RNA packaging
alphavirus
capsid protein
nucleocapsid
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
31 07 2021
31 07 2021
Historique:
received:
04
07
2021
revised:
26
07
2021
accepted:
28
07
2021
entrez:
28
8
2021
pubmed:
29
8
2021
medline:
3
2
2022
Statut:
epublish
Résumé
Alphaviruses are small enveloped viruses with positive-sense RNA genomes. During infection, the alphavirus capsid protein (Cp) selectively packages and assembles with the viral genomic RNA to form the nucleocapsid core, a process critical to the production of infectious virus. Prior studies of the alphavirus Semliki Forest virus (SFV) showed that packaging and assembly are promoted by Cp binding to multiple high affinity sites on the genomic RNA. Here, we developed an in vitro Cp binding assay based on fluorescently labeled RNA oligos. We used this assay to explore the RNA sequence and structure requirements for Cp binding to site #1, the top binding site identified on the genomic RNA during all stages of virus assembly. Our results identify a stem-loop structure that promotes specific binding of the SFV Cp to site #1 RNA. This structure is also recognized by the Cps of the related alphaviruses chikungunya virus and Ross River virus.
Identifiants
pubmed: 34452382
pii: v13081517
doi: 10.3390/v13081517
pmc: PMC8402798
pii:
doi:
Substances chimiques
Capsid Proteins
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI075647
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM122450
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013330
Pays : United States
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