Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression.
Adipose tissue
Lipogems
MSC
Microfragmentation
Osteoarthritis
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
28 08 2021
28 08 2021
Historique:
received:
27
11
2020
accepted:
02
08
2021
entrez:
29
8
2021
pubmed:
30
8
2021
medline:
30
10
2021
Statut:
epublish
Résumé
Adipose tissue (AT) has become a source of mesenchymal stromal/stem cells (MSC) for regenerative medicine applications, in particular skeletal disorders. Several enzymatic or mechanical procedures have been proposed to process AT with the aim to isolate cells that can be locally implanted. How AT is processed may impact its properties. Thus, we compared AT processed by centrifugation (C-AT) to microfragmentation (MF-AT). Focusing on MF-AT, we subsequently assessed the impact of synovial fluid (SF) alone on both MF-AT and isolated AT-MSC to better understand their cartilage repair mechanisms. MF-AT and C-AT from the same donors were compared by histology and qRT-PCR immediately after isolation or as ex vivo cultures using a micro-tissue pellet system. The in vitro impact of SF on MF-AT and AT-MSC was assessed by histological staining and molecular analysis. The main AT histological features (i.e., increased extracellular matrix and cellularity) of the freshly isolated or ex vivo-cultured MF-AT persisted compared to C-AT, which rapidly deteriorated during culture. Based on our previous studies of HOX genes in MSC, we investigated the involvement of Homeobox Protein HOX-B7 (HOXB7) and its target basic Fibroblast Growth Factor (bFGF) in the molecular mechanism underlying the improved performance of MF-AT. Indeed, both these biomarkers were more prominent in freshly isolated MF-AT compared to C-AT. SF alone preserved the AT histological features of MF-AT, together with HOXB7 and bFGF expression. Increased cell performance was also observed in isolated AT-MSC after SF treatment concomitant with enhanced HOXB7 expression, although there was no apparent association with bFGF. Our findings show that MF has a positive effect on the maintenance of AT histology and may trigger the expression of trophic factors that improve tissue repair by processed AT.
Identifiants
pubmed: 34454577
doi: 10.1186/s13287-021-02540-1
pii: 10.1186/s13287-021-02540-1
pmc: PMC8399787
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
481Informations de copyright
© 2021. The Author(s).
Références
Curr Stem Cell Rep. 2016;2:304-312
pubmed: 27547712
Stem Cell Res Ther. 2019 Mar 19;10(1):101
pubmed: 30890185
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Tissue Cell. 2004 Dec;36(6):431-8
pubmed: 15533458
Stem Cells Int. 2016;2016:1905846
pubmed: 26880936
Cell Regen. 2015 Sep 30;4:7
pubmed: 26435835
J Clin Endocrinol Metab. 2013 Jan;98(1):362-71
pubmed: 23150689
Nat Rev Mol Cell Biol. 2014 Oct;15(10):647-64
pubmed: 25237826
Chin J Traumatol. 2011;14(6):329-35
pubmed: 22152135
Sci Rep. 2017 Mar 23;7:45018
pubmed: 28332587
Knee Surg Sports Traumatol Arthrosc. 2021 Jan 19;:
pubmed: 33464397
Stem Cells Int. 2016;2016:4373410
pubmed: 27057170
Wiley Interdiscip Rev Syst Biol Med. 2012 Jan-Feb;4(1):15-37
pubmed: 21826801
Aging (Albany NY). 2011 Oct;3(10):920-33
pubmed: 21990129
Cytokine. 2013 Jan;61(1):1-14
pubmed: 23022179
Regen Med. 2020 Oct;15(10):2153-2161
pubmed: 33275470
Am J Orthop (Belle Mead NJ). 2018 Nov;47(11):
pubmed: 30517209
Osteoarthritis Cartilage. 2010 Nov;18(11):1441-7
pubmed: 20816981
J Craniofac Surg. 2015 May;26(3):756-9
pubmed: 25974786
Int J Mol Sci. 2019 Nov 02;20(21):
pubmed: 31684107
Organogenesis. 2009 Apr;5(2):50-6
pubmed: 19794899
Plast Reconstr Surg. 2006 Sep;118(3 Suppl):108S-120S
pubmed: 16936550
Tissue Eng. 2001 Apr;7(2):211-28
pubmed: 11304456
Genes (Basel). 2017 Oct 13;8(10):
pubmed: 29027984
Cell Transplant. 2013;22(11):2063-77
pubmed: 23051701
Stem Cells. 2015 Mar;33(3):939-50
pubmed: 25428821
Cytotherapy. 2013 Jun;15(6):641-8
pubmed: 23570660
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10261-6
pubmed: 9294198
J Exp Orthop. 2017 Oct 3;4(1):33
pubmed: 28975547
Gerontology. 2012;58(1):15-23
pubmed: 21135534
Obes Res. 2002 Jul;10(7):608-16
pubmed: 12105282
Cell Mol Life Sci. 2002 Aug;59(8):1317-26
pubmed: 12363035
Aging Dis. 2020 May 9;11(3):465-469
pubmed: 32489692
Wideochir Inne Tech Maloinwazyjne. 2015 Jul;10(2):337-41
pubmed: 26240640
Int J Mol Sci. 2018 Jan 16;19(1):
pubmed: 29337886
BMC Musculoskelet Disord. 2018 May 30;19(1):176
pubmed: 29848328
Aesthetic Plast Surg. 2014 Oct;38(5):985-93
pubmed: 25053112
Apoptosis. 2013 Oct;18(10):1274-89
pubmed: 23828239
Int Orthop. 2013 Jan;37(1):145-51
pubmed: 23212731
Int J Biochem Cell Biol. 2004 Aug;36(8):1424-39
pubmed: 15147722
Biorheology. 2002;39(1-2):237-46
pubmed: 12082286
J Cell Physiol. 2000 Mar;182(3):311-22
pubmed: 10653597
FASEB J. 2006 Aug;20(10):1730-2
pubmed: 16790525
Cell Transplant. 2016;25(6):1043-56
pubmed: 26395761
Children (Basel). 2020 Oct 13;7(10):
pubmed: 33066133
Mol Cell Biol. 1996 Sep;16(9):4842-51
pubmed: 8756643
Stem Cells Int. 2020 Aug 4;2020:8881405
pubmed: 32831853
Dan Med J. 2019 Oct;66(10):
pubmed: 31571571
Biomaterials. 2012 Mar;33(7):2086-96
pubmed: 22169138
Stem Cells. 2017 Jan;35(1):117-134
pubmed: 27641055