Hook-basal-body assembly state dictates substrate specificity of the flagellar type-III secretion system.
flagella biosynthesis
motility
protein transport
substrate specificity switching
type-III secretion
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
27
08
2021
received:
18
05
2021
accepted:
28
08
2021
pubmed:
2
9
2021
medline:
22
2
2022
entrez:
1
9
2021
Statut:
ppublish
Résumé
The assembly of the bacterial flagellum is orchestrated by the secretion of distinct early and late secretion substrates via the flagellar-specific type-III secretion system (fT3SS). However, how the fT3SS is able to distinguish between the different (early and late) substrate classes during flagellar assembly remains poorly understood. In this study, we investigated the substrate selectivity and specificity of the fT3SS of Salmonella enterica at different assembly stages. For this, we developed an experimental setup that allowed us to synchronize hook-basal-body assembly and to monitor early and late substrate secretion of fT3SSs operating in either early or late secretion mode, respectively. Our results demonstrate that the fT3SS features a remarkable specificity for only the substrates required at the respective assembly stage. No crosstalk of substrates was observed for fT3SSs operating in the opposing secretion mode. We further found that a substantial fraction of fT3SS surprisingly remained in early secretion mode. Our results thus suggest that the secretion substrate specificity switch of the fT3SS is unidirectional and irreversible. The developed secretion substrate reporter system further provides a platform for future investigations of the underlying molecular mechanisms of the elusive substrate recognition of the T3SS.
Substances chimiques
Bacterial Proteins
0
Membrane Proteins
0
Type III Secretion Systems
0
hook protein, bacterial flagellum
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1189-1200Informations de copyright
© 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.
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