In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography.
bacteria
cryo-ET
infectious disease
membrane extensions
microbiology
secretin
tubes
vesicles
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
01 09 2021
01 09 2021
Historique:
received:
17
08
2021
accepted:
23
08
2021
pubmed:
2
9
2021
medline:
21
10
2021
entrez:
1
9
2021
Statut:
epublish
Résumé
The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: (1) tubes with a uniform diameter (with or without an internal scaffold), (2) tubes with irregular diameter, (3) tubes with a vesicular dilation at their tip, (4) pearling tubes, (5) connected chains of vesicles (with or without neck-like connectors), (6) budding vesicles and nanopods. We also identified several protein complexes associated with these MEs and MVs which were distributed either randomly or exclusively at the tip. These complexes include a secretin-like structure and a novel crown-shaped structure observed primarily in vesicles from lysed cells. In total, this work helps to characterize the diversity of bacterial membrane projections and lays the groundwork for future research in this field.
Identifiants
pubmed: 34468314
doi: 10.7554/eLife.73099
pii: 73099
pmc: PMC8455137
doi:
pii:
Substances chimiques
Bacterial Outer Membrane Proteins
0
Multiprotein Complexes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM130456
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122588
Pays : United States
Informations de copyright
© 2021, Kaplan et al.
Déclaration de conflit d'intérêts
MK, LM, JL, CW, CO, WN, PS, LZ, YW, YC, MB, MD, YZ, MM, AB, CS, GJ none, GC None
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