ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor.
CCW
CW
CheY
flagellum
motor
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
30
08
2021
received:
05
08
2021
accepted:
01
09
2021
pubmed:
7
9
2021
medline:
14
1
2022
entrez:
6
9
2021
Statut:
ppublish
Résumé
Bacteria exhibit chemotaxis by controlling flagellar rotation to move toward preferred places or away from nonpreferred places. The change in rotation is triggered by the binding of the chemotaxis signaling protein CheY-phosphate (CheY-P) to the C-ring in the flagellar motor. Some specific bacteria, including Vibrio spp. and Shewanella spp., have a single transmembrane protein called ZomB. ZomB is essential for controlling the flagellar rotational direction in Shewanella putrefaciens and Vibrio parahaemolyticus. In this study, we confirmed that the zomB deletion results only in the counterclockwise (CCW) rotation of the motor in Vibrio alginolyticus as previously reported in other bacteria. We found that ZomB is not required for a clockwise-locked phenotype caused by mutations in fliG and fliM, and that ZomB is essential for CW rotation induced by overproduction of CheY-P. Purified ZomB proteins form multimers, suggesting that ZomB may function as a homo-oligomer. These observations imply that ZomB interacts with protein(s) involved in either flagellar motor rotation, chemotaxis, or both. We provide the evidence that ZomB is a new player in chemotaxis and is required for the rotational control in addition to CheY in Vibrio alginolyticus.
Substances chimiques
Bacterial Proteins
0
Escherichia coli Proteins
0
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
927-937Subventions
Organisme : JSPS KAKENHI
ID : JP16J01859
Organisme : JSPS KAKENHI
ID : JP20J00329
Organisme : JSPS KAKENHI
ID : JP20H03220
Informations de copyright
© 2021 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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