Genetically encoded cell-death indicators (GEDI) to detect an early irreversible commitment to neurodegeneration.
Animals
Biosensing Techniques
Calcium
/ metabolism
Cell Death
/ genetics
Cerebral Cortex
/ cytology
DNA-Binding Proteins
/ genetics
Disease Models, Animal
Embryo, Nonmammalian
Fluorescent Dyes
/ chemistry
Gene Expression Regulation, Developmental
Genes, Reporter
Glutamic Acid
/ pharmacology
Green Fluorescent Proteins
/ genetics
Humans
Larva
/ cytology
Mice
Mice, Inbred C57BL
Neurodegenerative Diseases
/ genetics
Neurons
/ cytology
Primary Cell Culture
Rats
Rats, Long-Evans
Single-Cell Analysis
/ methods
Superoxide Dismutase-1
/ genetics
Zebrafish
/ embryology
alpha-Synuclein
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 09 2021
06 09 2021
Historique:
received:
18
12
2019
accepted:
16
08
2021
entrez:
7
9
2021
pubmed:
8
9
2021
medline:
6
10
2021
Statut:
epublish
Résumé
Cell death is a critical process that occurs normally in health and disease. However, its study is limited due to available technologies that only detect very late stages in the process or specific death mechanisms. Here, we report the development of a family of fluorescent biosensors called genetically encoded death indicators (GEDIs). GEDIs specifically detect an intracellular Ca
Identifiants
pubmed: 34489414
doi: 10.1038/s41467-021-25549-9
pii: 10.1038/s41467-021-25549-9
pmc: PMC8421388
doi:
Substances chimiques
DNA-Binding Proteins
0
Fluorescent Dyes
0
SOD1 protein, human
0
TARDBP protein, human
0
alpha-Synuclein
0
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Glutamic Acid
3KX376GY7L
Superoxide Dismutase-1
EC 1.15.1.1
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5284Subventions
Organisme : NIA NIH HHS
ID : RF1 AG064170
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH115747
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG056151
Pays : United States
Organisme : NIA NIH HHS
ID : P01 AG054407
Pays : United States
Organisme : NINDS NIH HHS
ID : U54 NS091046
Pays : United States
Organisme : NCRR NIH HHS
ID : C06 RR018928
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG058476
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG058447
Pays : United States
Organisme : NINDS NIH HHS
ID : R37 NS101996
Pays : United States
Organisme : NLM NIH HHS
ID : R01 LM013617
Pays : United States
Informations de copyright
© 2021. The Author(s).
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