Spontaneously Resolving Joint Inflammation Is Characterised by Metabolic Agility of Fibroblast-Like Synoviocytes.
arthritis
fibroblasts
inflammation
metabolism
mitochondria
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
15
06
2021
accepted:
09
08
2021
entrez:
13
9
2021
pubmed:
14
9
2021
medline:
12
1
2022
Statut:
epublish
Résumé
Fibroblast-like synoviocytes (FLS) play an important role in maintaining joint homeostasis and orchestrating local inflammatory processes. When activated during injury or inflammation, FLS undergo transiently increased bioenergetic and biosynthetic demand. We aimed to identify metabolic changes which occur early in inflammatory disease pathogenesis which might support sustained cellular activation in persistent inflammation. We took primary human FLS from synovial biopsies of patients with very early rheumatoid arthritis (veRA) or resolving synovitis, and compared them with uninflamed control samples from the synovium of people without arthritis. Metabotypes were compared using NMR spectroscopy-based metabolomics and correlated with serum C-reactive protein levels. We measured glycolysis and oxidative phosphorylation by Seahorse analysis and assessed mitochondrial morphology by immunofluorescence. We demonstrate differences in FLS metabolism measurable after
Identifiants
pubmed: 34512657
doi: 10.3389/fimmu.2021.725641
pmc: PMC8426599
doi:
Substances chimiques
TNF protein, human
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
725641Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Versus Arthritis
ID : 22072
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : 19791
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : 20298
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S025308/1
Pays : United Kingdom
Organisme : Versus Arthritis
ID : 21802
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : 20015
Pays : United Kingdom
Informations de copyright
Copyright © 2021 Falconer, Pucino, Clayton, Marshall, Raizada, Adams, Philp, Clark, Filer, Raza, Young and Buckley.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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