Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species.
Animals
Cell Line
Databases, Genetic
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ enzymology
Gene Expression Regulation, Fungal
Humans
Mediator Complex
/ chemistry
Mice
Promoter Regions, Genetic
Protein Conformation
RNA Polymerase II
/ chemistry
Saccharomyces cerevisiae
/ enzymology
Saccharomyces cerevisiae Proteins
/ genetics
Species Specificity
Structure-Activity Relationship
TATA-Binding Protein Associated Factors
/ genetics
TATA-Box Binding Protein
/ genetics
Transcription Factors, General
/ chemistry
Transcription Initiation Site
Transcription Initiation, Genetic
D. melanogaster
Mediator
RNA polymerase II
S. cerevisiae
S. pombe
TFIID
chromosomes
gene expression
genetics
genomics
human
mouse
promoters
transcription factors
transcriptional initiation
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
13 09 2021
13 09 2021
Historique:
received:
01
03
2021
accepted:
10
09
2021
pubmed:
14
9
2021
medline:
21
10
2021
entrez:
13
9
2021
Statut:
epublish
Résumé
The preinitiation complex (PIC) for transcriptional initiation by RNA polymerase (Pol) II is composed of general transcription factors that are highly conserved. However, analysis of ChIP-seq datasets reveals kinetic and compositional differences in the transcriptional initiation process among eukaryotic species. In yeast, Mediator associates strongly with activator proteins bound to enhancers, but it transiently associates with promoters in a form that lacks the kinase module. In contrast, in human, mouse, and fly cells, Mediator with its kinase module stably associates with promoters, but not with activator-binding sites. This suggests that yeast and metazoans differ in the nature of the dynamic bridge of Mediator between activators and Pol II and the composition of a stable inactive PIC-like entity. As in yeast, occupancies of TATA-binding protein (TBP) and TBP-associated factors (Tafs) at mammalian promoters are not strictly correlated. This suggests that within PICs, TFIID is not a monolithic entity, and multiple forms of TBP affect initiation at different classes of genes. TFIID in flies, but not yeast and mammals, interacts strongly at regions downstream of the initiation site, consistent with the importance of downstream promoter elements in that species. Lastly, Taf7 and the mammalian-specific Med26 subunit of Mediator also interact near the Pol II pause region downstream of the PIC, but only in subsets of genes and often not together. Species-specific differences in PIC structure and function are likely to affect how activators and repressors affect transcriptional activity.
Identifiants
pubmed: 34515029
doi: 10.7554/eLife.67964
pii: 67964
pmc: PMC8463073
doi:
pii:
Substances chimiques
Drosophila Proteins
0
Mediator Complex
0
Saccharomyces cerevisiae Proteins
0
TATA-Binding Protein Associated Factors
0
TATA-Box Binding Protein
0
Transcription Factors, General
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM030186
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131801
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM030186
Pays : United States
Informations de copyright
© 2021, Petrenko and Struhl.
Déclaration de conflit d'intérêts
NP none, KS Senior editor, eLife
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