Importin α2 association with chromatin: Direct DNA binding via a novel DNA-binding domain.
DNA-binding protein
chromatin
importin α
nuclear transport
nucleus
protein−DNA interaction
α-helix
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
revised:
10
09
2021
received:
18
08
2021
accepted:
11
09
2021
pubmed:
15
9
2021
medline:
14
1
2022
entrez:
14
9
2021
Statut:
ppublish
Résumé
The nuclear transport of proteins is important for facilitating appropriate nuclear functions. The importin α family proteins play key roles in nuclear transport as transport receptors for copious nuclear proteins. Additionally, these proteins possess other functions, including chromatin association and gene regulation. However, these nontransport functions of importin α are not yet fully understood, especially their molecular-level mechanisms and consequences for functioning with chromatin. Here, we report the novel molecular characteristics of importin α binding to diverse DNA sequences in chromatin. We newly identified and characterized a DNA-binding domain-the Nucleic Acid Associating Trolley pole domain (NAAT domain)-in the N-terminal region of importin α within the conventional importin β binding (IBB) domain that is necessary for nuclear transport of cargo proteins. Furthermore, we found that the DNA binding of importin α synergistically coupled the recruitment of its cargo protein to DNA. This is the first study to delineate the interaction between importin α and chromatin DNA via the NAAT domain, indicating the bifunctionality of the importin α N-terminal region for nuclear transport and chromatin association.
Substances chimiques
Chromatin
0
Nuclear Localization Signals
0
Nuclear Proteins
0
alpha Karyopherins
0
karyopherin alpha 2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
945-966Subventions
Organisme : Nihon University
Organisme : JSPS
ID : KAKENHI:18H04870
Organisme : JSPS
ID : 15K07069
Organisme : JSPS
ID : 25116008
Organisme : JSPS
ID : 18H05531
Organisme : JSPS
ID : 18K
Informations de copyright
© 2021 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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