Historical, current, and emerging tools for identification and serotyping of Shigella.
Escherichia coli
Identification
Serotyping
Shigella spp.
Whole-genome sequencing
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
28
12
2020
accepted:
29
06
2021
pubmed:
16
9
2021
medline:
16
12
2021
entrez:
15
9
2021
Statut:
ppublish
Résumé
The Shigella genus includes serious foodborne disease etiologic agents, with 4 species and 54 serotypes. Identification at species and serotype levels is a crucial task in microbiological laboratories. Nevertheless, the genetic similarity between Shigella spp. and Escherichia coli challenges the correct identification and serotyping of Shigella spp., with subsequent negative repercussions on surveillance, epidemiological investigations, and selection of appropriate treatments. For this purpose, multiple techniques have been developed historically ranging from phenotype-based methods and single or multilocus molecular techniques to whole-genome sequencing (WGS). To facilitate the selection of the most relevant method, we herein provide a global overview of historical and emerging identification and serotyping techniques with a particular focus on the WGS-based approaches. This review highlights the excellent discriminatory power of WGS to more accurately elucidate the epidemiology of Shigella spp., disclose novel promising genomic targets for surveillance methods, and validate previous well-established methods.
Identifiants
pubmed: 34524650
doi: 10.1007/s42770-021-00573-5
pii: 10.1007/s42770-021-00573-5
pmc: PMC8441030
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
2043-2055Informations de copyright
© 2021. Sociedade Brasileira de Microbiologia.
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