Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae.
Cell wall integrity
Endocytosis
Magnaporthe oryzae
Momyo1
Pathogenicity
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
28
01
2021
accepted:
25
08
2021
revised:
23
08
2021
pubmed:
19
9
2021
medline:
9
10
2021
entrez:
18
9
2021
Statut:
ppublish
Résumé
In eukaryotes, myosin provides the necessary impetus for a series of physiological processes, including organelle movement, cytoplasmic flow, cell division, and mitosis. Previously, three members of myosin were identified in Magnaporthe oryzae, with class II and class V myosins playing important roles in intracellular transport, fungal growth, and pathogenicity. However, limited is known about the biological function of the class I myosin protein in the rice blast fungus. Here, we found that Momyo1 is highly expressed during conidiation and infection. Functional characterization of this gene via RNA interference (RNAi) revealed that Momyo1 is required for vegetative growth, conidiation, melanin pigmentation, and pathogenicity of M. oryzae. The Momyo1 knockdown mutant is defective in formation of appressorium-like structures (ALS) at the hyphal tips. In addition, Momyo1 also displays defects on cell wall integrity, hyphal hydrophobicity, extracellular enzyme activities, endocytosis, and formation of the Spitzenkörper. Furthermore, Momyo1 was identified to physically interact with the MoShe4, a She4p/Dim1p orthologue potentially involved in endocytosis, polarization of the actin cytoskeleton. Overall, our findings provide a novel insight into the regulatory mechanism of Momyo1 that is involved in fungal growth, cell wall integrity, endocytosis, and virulence of M. oryzae. KEY POINTS: • Momyo1 is required for vegetative growth and pigmentation of M. oryzae. • Momyo1 is essential for cell wall integrity and endocytosis of M. oryzae. • Momyo1 is involved in hyphal surface hydrophobicity of M. oryzae.
Identifiants
pubmed: 34536105
doi: 10.1007/s00253-021-11573-8
pii: 10.1007/s00253-021-11573-8
doi:
Substances chimiques
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7395-7410Subventions
Organisme : Wellcome Trust
ID : 201470
Pays : United Kingdom
Organisme : National Natural Science Foundation of China
ID : 31671976
Organisme : Foundation for the Author of National Excellent Doctoral Dissertation of the People's Republic of China (CN)
ID : RC2015002
Organisme : Anhui Agricultural University Postgraduate Innovation Foundation
ID : 2020ysj-8
Organisme : Wellcome Trust
ID : 201470
Pays : United Kingdom
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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