Enantioselective multiple heart cutting online two-dimensional liquid chromatography-mass spectrometry of all proteinogenic amino acids with second dimension chiral separations in one-minute time scales on a chiral tandem column.
6-Aminoquinolyl-N-Hydroxysuccinimidyl carbamate
Enantioselective amino acid separation
L-[u-(13)C(15)N] amino acid internal standard
Multiple heart-cutting
Two-dimensional liquid chromatography
UHPLC
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
02 Oct 2021
02 Oct 2021
Historique:
received:
07
06
2021
revised:
15
07
2021
accepted:
16
07
2021
entrez:
20
9
2021
pubmed:
21
9
2021
medline:
22
9
2021
Statut:
ppublish
Résumé
In this work, we present a unique, robust and fully automated analytical platform technology for the enantioselective amino acid analysis using a multiple heart cutting RPLC-enantio/stereoselective HPLC-ESI-QTOF-MS method. This 2D-LC method allows the full enantioselective separation of 20 proteinogenic AAs plus 5 isobaric analogues, namely allo-Threonine (aThr), homoserine (Hse), allo-isoleucine (aIle), tert-Leucine (Tle) and Norleucine (Nle), after pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC; AccQ). This N-terminal AA-derivatization method introduces on the one hand beneficial chromatographic properties for
Identifiants
pubmed: 34538327
pii: S0003-2670(21)00684-X
doi: 10.1016/j.aca.2021.338858
pii:
doi:
Substances chimiques
Amino Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
338858Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.