Estrogen Replacement Therapy Induces Antioxidant and Longevity-Related Genes in Women after Medically Induced Menopause.
Adolescent
Adult
Antioxidants
/ metabolism
Estradiol
/ administration & dosage
Estrogen Replacement Therapy
/ methods
Estrogens
/ blood
Female
Glutathione
/ blood
Glutathione Peroxidase
/ genetics
Humans
Leukocytes, Mononuclear
/ cytology
Longevity
/ genetics
Menopause
/ drug effects
Progesterone
/ blood
RNA, Ribosomal, 16S
/ genetics
Superoxide Dismutase
/ genetics
Telomeric Repeat Binding Protein 2
/ genetics
Triptorelin Pamoate
/ pharmacology
Up-Regulation
/ drug effects
Young Adult
Journal
Oxidative medicine and cellular longevity
ISSN: 1942-0994
Titre abrégé: Oxid Med Cell Longev
Pays: United States
ID NLM: 101479826
Informations de publication
Date de publication:
2021
2021
Historique:
received:
16
04
2021
accepted:
25
08
2021
entrez:
20
9
2021
pubmed:
21
9
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Females live longer than males in many species, including humans, and estrogens are in part responsible for this protection against aging. We reported previously that estrogens can protect rats against oxidative stress, by inducing antioxidant and longevity-related genes. Thus, this study was aimed at confirming the ability of estrogens to upregulate antioxidant and longevity-related genes in humans. For this purpose, we selected 16 women of reproductive age (18-42 years old) undergoing a fertility treatment that includes a medically induced menopause, at the Valencian Infertility Institute. We took blood samples at each time point of the treatment (basal, induced menopause, estrogen, and estrogen plus progesterone replacement therapy). mRNA expression of antioxidant and longevity-related genes in peripheral blood mononuclear cells (PBMC) was determined by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Determination of reduced glutathione (GSH) in total blood was carried out using high-performance liquid chromatography (HPLC). As expected, we found that medically induced menopause significantly decreased sexual hormone (estrogens and progesterone) levels. It also lowered glutathione peroxidase (GPx), 16S rRNA, P21, and TERF2 mRNA expression and blood GSH levels. Estrogen replacement therapy significantly restored estrogen levels and induced mRNA expression of manganese superoxide dismutase (MnSOD), GPx, 16S rRNA, P53, P21, and TERF2 and restored blood GSH levels. Progesterone replacement therapy induced a significant increase in MnSOD, P53, sestrin 2 (SENS2), and TERF2 mRNA expression when compared to basal conditions. These findings provide evidence for estrogen beneficial effects in upregulating antioxidant and longevity-related genes in women.
Identifiants
pubmed: 34539974
doi: 10.1155/2021/8101615
pmc: PMC8448598
doi:
Substances chimiques
Antioxidants
0
Estrogens
0
RNA, Ribosomal, 16S
0
Telomeric Repeat Binding Protein 2
0
Triptorelin Pamoate
08AN7WA2G0
Progesterone
4G7DS2Q64Y
Estradiol
4TI98Z838E
Glutathione Peroxidase
EC 1.11.1.9
Superoxide Dismutase
EC 1.15.1.1
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8101615Informations de copyright
Copyright © 2021 C. Borrás et al.
Déclaration de conflit d'intérêts
The authors declare that they have no financial/commercial conflict of interests regarding the publication of this paper.
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