Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.
C. elegans
embryogenesis
essential genes
fertilization
legacy mutants
maternal-effect
whole-genome sequencing
Journal
G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598
Informations de publication
Date de publication:
08 12 2021
08 12 2021
Historique:
accepted:
27
08
2021
received:
16
06
2021
pubmed:
23
9
2021
medline:
8
1
2022
entrez:
22
9
2021
Statut:
ppublish
Résumé
It has been estimated that 15%-30% of the ∼20,000 genes in C. elegans are essential, yet many of these genes remain to be identified or characterized. With the goal of identifying unknown essential genes, we performed whole-genome sequencing on complementation pairs from legacy collections of maternal-effect lethal and sterile mutants. This approach uncovered maternal genes required for embryonic development and genes with apparent sperm-specific functions. In total, 58 putative essential genes were identified on chromosomes III-V, of which 52 genes are represented by novel alleles in this collection. Of these 52 genes, 19 (40 alleles) were selected for further functional characterization. The terminal phenotypes of embryos were examined, revealing defects in cell division, morphogenesis, and osmotic integrity of the eggshell. Mating assays with wild-type males revealed previously unknown male-expressed genes required for fertilization and embryonic development. The result of this study is a catalog of mutant alleles in essential genes that will serve as a resource to guide further study toward a more complete understanding of this important model organism. As many genes and developmental pathways in C. elegans are conserved and essential genes are often linked to human disease, uncovering the function of these genes may also provide insight to further our understanding of human biology.
Identifiants
pubmed: 34550348
pii: 6373896
doi: 10.1093/g3journal/jkab328
pmc: PMC8664450
pii:
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Banques de données
figshare
['10.25386/genetics.14702139']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : R24 OD023041
Pays : United States
Organisme : CIHR
ID : PJT-148549
Pays : Canada
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.
Références
Genetics. 1988 Dec;120(4):977-86
pubmed: 3224814
Dev Cell. 2016 Aug 22;38(4):430-44
pubmed: 27554860
Cell. 2011 Apr 29;145(3):470-82
pubmed: 21529718
Nat Methods. 2008 Feb;5(2):183-8
pubmed: 18204455
BMC Genomics. 2009 Nov 16;10:529
pubmed: 19917126
Methods Mol Biol. 2009;518:123-33
pubmed: 19085141
Science. 1996 Aug 2;273(5275):603-7
pubmed: 8662545
Nat Biotechnol. 2011 Apr;29(4):361-7
pubmed: 21441928
Dev Cell. 2009 Oct;17(4):494-504
pubmed: 19853563
J Cell Sci. 2004 Sep 1;117(Pt 19):4571-82
pubmed: 15331665
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
G3 (Bethesda). 2012 Nov;2(11):1415-25
pubmed: 23173093
Genetics. 1995 Nov;141(3):1101-11
pubmed: 8582616
Trends Parasitol. 2001 Aug;17(8):387-93
pubmed: 11685900
Nature. 2014 Aug 28;512(7515):445-8
pubmed: 25164755
Genome Res. 2016 Oct;26(10):1441-1450
pubmed: 27531719
Genetics. 1991 Nov;129(3):735-52
pubmed: 1752418
Genome Res. 2013 Apr;23(4):727-35
pubmed: 23382537
Mol Gen Genet. 1998 Nov;260(2-3):280-8
pubmed: 9862482
J Cell Biol. 1999 Mar 8;144(5):927-46
pubmed: 10085292
PLoS One. 2010 Nov 08;5(11):e15435
pubmed: 21079745
Genetics. 1978 Jan;88(1):49-65
pubmed: 631558
Dev Cell. 2001 Sep;1(3):363-75
pubmed: 11702948
Nature. 2000 Nov 16;408(6810):325-30
pubmed: 11099033
J Biol Chem. 1998 Mar 13;273(11):6066-73
pubmed: 9497323
Genetics. 2010 Sep;186(1):427-30
pubmed: 20610404
Genes Dev. 1999 Nov 1;13(21):2838-51
pubmed: 10557211
G3 (Bethesda). 2019 Jan 9;9(1):135-144
pubmed: 30420468
Nat Rev Genet. 2010 Jan;11(1):31-46
pubmed: 19997069
Nature. 2000 Nov 16;408(6810):331-6
pubmed: 11099034
Life Sci. 2006 Nov 25;79(26):2413-31
pubmed: 16979192
PLoS Biol. 2006 Nov;4(12):e396
pubmed: 17121454
BMC Genomics. 2014 Feb 28;15:168
pubmed: 24581041
Genetics. 1982 Dec;102(4):725-36
pubmed: 7187364
Angew Chem Int Ed Engl. 2017 Jun 1;56(23):6396-6419
pubmed: 27943572
Sci Adv. 2015;1(3):e1500248
pubmed: 25977940
BMC Dev Biol. 2003 Oct 03;3:8
pubmed: 14527340
Genetics. 2010 Jun;185(2):431-41
pubmed: 20439774
Development. 2004 Jan;131(2):311-23
pubmed: 14668411
Nature. 2005 Mar 24;434(7032):462-9
pubmed: 15791247
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2707-12
pubmed: 16477039
Mol Biol Cell. 2009 Feb;20(4):1223-40
pubmed: 19109425
WormBook. 2018 Aug 2;2018:1-36
pubmed: 26715360
G3 (Bethesda). 2016 May 03;6(5):1297-304
pubmed: 26945029
Genome Res. 2008 Oct;18(10):1638-42
pubmed: 18775913
Pediatr Res. 2009 Jan;65(1):10-8
pubmed: 18852689
Genetics. 2015 Oct;201(2):449-58
pubmed: 26232410
PLoS Biol. 2004 Sep;2(9):E257
pubmed: 15340492
Dev Biol. 2009 Sep 15;333(2):397-408
pubmed: 19591818
PLoS Genet. 2008 Aug 01;4(8):e1000139
pubmed: 18670626
Genetics. 1979 May;92(1):99-115
pubmed: 574105
PLoS One. 2011;6(9):e24656
pubmed: 21935434
Nat Rev Genet. 2016 May 17;17(6):333-51
pubmed: 27184599
PLoS One. 2011;6(11):e27368
pubmed: 22096564
Genetics. 1990 Nov;126(3):583-92
pubmed: 2249758
Genetics. 1988 Jun;119(2):345-53
pubmed: 3396868
Nature. 2003 Jan 16;421(6920):231-7
pubmed: 12529635
Nat Methods. 2009 Aug;6(8):550-1
pubmed: 19644454
J Cell Biol. 2012 Aug 20;198(4):731-48
pubmed: 22908315
Mol Gen Genet. 1992 May;233(1-2):241-51
pubmed: 1603066
BMC Genomics. 2010 Apr 03;11:222
pubmed: 20361874
Nat Biotechnol. 2013 Apr;31(4):325-30
pubmed: 23475072
Trends Parasitol. 2004 Oct;20(10):469-76
pubmed: 15363440
Mol Biol Cell. 2006 Nov;17(11):4736-47
pubmed: 16943325
Nature. 1998 Feb 19;391(6669):806-11
pubmed: 9486653
J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7
pubmed: 20519582
Genetics. 1974 May;77(1):71-94
pubmed: 4366476
Nature. 2002 Jul 25;418(6896):387-91
pubmed: 12140549
Genome Res. 2009 Apr;19(4):657-66
pubmed: 19181841
Genetics. 1999 Jan;151(1):131-41
pubmed: 9872954
EMBO J. 1991 Dec;10(12):3959-70
pubmed: 1935914
Genome Res. 2013 Oct;23(10):1749-62
pubmed: 23800452
Nature. 2004 Aug 5;430(7000):679-82
pubmed: 15295601
J Cell Sci. 2002 Jun 1;115(Pt 11):2293-302
pubmed: 12006614
Development. 2004 Aug;131(15):3527-43
pubmed: 15215208
G3 (Bethesda). 2018 Mar 2;8(3):981-997
pubmed: 29339407
Science. 2018 May 4;360(6388):
pubmed: 29724925
Int J Parasitol. 1998 Mar;28(3):395-411
pubmed: 9559358
BMC Biol. 2006 Oct 16;4:35
pubmed: 17042944
Trends Plant Sci. 2011 May;16(5):282-8
pubmed: 21439889
Science. 1999 Aug 6;285(5429):901-6
pubmed: 10436161
Development. 2019 Apr 11;146(7):
pubmed: 30890570
Science. 1998 Dec 11;282(5396):2012-8
pubmed: 9851916
Mutat Res. 1988 Sep;201(1):137-47
pubmed: 3419443
Hum Mol Genet. 2000 Apr 12;9(6):869-77
pubmed: 10767309
Funct Integr Genomics. 2002 Sep;2(4-5):199-211
pubmed: 12192593
PLoS Genet. 2013 May;9(5):e1003484
pubmed: 23675308
PLoS One. 2007 Nov 14;2(11):e1189
pubmed: 18000556
Genetics. 2018 Oct;210(2):445-461
pubmed: 30120140
Science. 2010 Dec 24;330(6012):1775-87
pubmed: 21177976
Dev Biol. 1997 Apr 15;184(2):234-65
pubmed: 9133433
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Nat Biotechnol. 2015 Jun;33(6):661-7
pubmed: 25961408
Mamm Genome. 2012 Oct;23(9-10):580-6
pubmed: 22968824
Curr Biol. 2003 May 13;13(10):819-27
pubmed: 12747829
Genetics. 2009 May;182(1):25-32
pubmed: 19307605
Genome Res. 2002 Jun;12(6):962-8
pubmed: 12045149
Genome Res. 2003 Sep;13(9):2129-41
pubmed: 12952881
Science. 2019 Sep 20;365(6459):
pubmed: 31488706
Proc Natl Acad Sci U S A. 1984 Feb;81(3):819-23
pubmed: 6583682
Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2602-7
pubmed: 24550289
PLoS Genet. 2014 Nov 20;10(11):e1004777
pubmed: 25412110
G3 (Bethesda). 2015 Mar 04;5(5):1007-19
pubmed: 25740937
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Genetics. 1988 Jan;118(1):49-59
pubmed: 8608931
Nat Methods. 2008 Oct;5(10):865-7
pubmed: 18677319
J Clin Invest. 2008 Apr;118(4):1311-21
pubmed: 18382743
Mech Dev. 2009 May-Jun;126(5-6):382-93
pubmed: 19368796
Nat Cell Biol. 2004 Jul;6(7):656-64
pubmed: 15232593
Worm. 2016 Sep 19;5(4):e1234659
pubmed: 28090394