Novel putative polyethylene terephthalate (PET) plastic degrading enzymes from the environmental metagenome.
PETase
environmental microbiome
plastic degradation
plastic recycling
polyethylene terephthalate degradation
Journal
Proteins
ISSN: 1097-0134
Titre abrégé: Proteins
Pays: United States
ID NLM: 8700181
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
revised:
02
08
2021
received:
04
12
2020
accepted:
18
08
2021
pubmed:
24
9
2021
medline:
15
3
2022
entrez:
23
9
2021
Statut:
ppublish
Résumé
Several plastic degrading enzymes have been described in the literature, most notably PETases that are capable of hydrolyzing polyethylene terephthalate (PET) plastic. One of them, the PETase from Ideonella sakaiensis, a bacterium isolated from environmental samples within a PET bottle recycling site, was a subject of extensive studies. To test how widespread PETase functionality is in other bacterial communities, we used a cascade of BLAST searches in the JGI metagenomic datasets and showed that close homologs of I. sakaiensis PETase can also be found in other metagenomic environmental samples from both human-affected and relatively pristine sites. To confirm their classification as putative PETases, we verified that the newly identified proteins have the PETase sequence signatures common to known PETases and that phylogenetic analyses group them with the experimentally characterized PETases. Additionally, docking analysis was performed in order to further confirm the functional assignment of the putative environmental PETases.
Identifiants
pubmed: 34553433
doi: 10.1002/prot.26245
pmc: PMC9524616
mid: NIHMS1742181
doi:
Substances chimiques
Bacterial Proteins
0
Plastics
0
Polyethylene Terephthalates
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
504-511Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201700060C
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118187
Pays : United States
Informations de copyright
© 2021 Wiley Periodicals LLC.
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