Coupling of spliceosome complexity to intron diversity.
Cryptococcus neoformans
RNA
Splicing
evolution
fidelity
spliceosome
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
22 11 2021
22 11 2021
Historique:
received:
26
04
2021
revised:
17
08
2021
accepted:
01
09
2021
pubmed:
24
9
2021
medline:
7
4
2022
entrez:
23
9
2021
Statut:
ppublish
Résumé
We determined that over 40 spliceosomal proteins are conserved between many fungal species and humans but were lost during the evolution of S. cerevisiae, an intron-poor yeast with unusually rigid splicing signals. We analyzed null mutations in a subset of these factors, most of which had not been investigated previously, in the intron-rich yeast Cryptococcus neoformans. We found they govern splicing efficiency of introns with divergent spacing between intron elements. Importantly, most of these factors also suppress usage of weak nearby cryptic/alternative splice sites. Among these, orthologs of GPATCH1 and the helicase DHX35 display correlated functional signatures and copurify with each other as well as components of catalytically active spliceosomes, identifying a conserved G patch/helicase pair that promotes splicing fidelity. We propose that a significant fraction of spliceosomal proteins in humans and most eukaryotes are involved in limiting splicing errors, potentially through kinetic proofreading mechanisms, thereby enabling greater intron diversity.
Identifiants
pubmed: 34555349
pii: S0960-9822(21)01244-6
doi: 10.1016/j.cub.2021.09.004
pmc: PMC8967684
mid: NIHMS1743503
pii:
doi:
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4898-4910.e4Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM071801
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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