A novel approach to imaging and visualization of minute amounts of DNA in small volume samples.

DNA / genetics Intercalating Agents Nucleic Acid Amplification Techniques

Journal

The Analyst
ISSN: 1364-5528
Titre abrégé: Analyst
Pays: England
ID NLM: 0372652

Informations de publication

Date de publication:
25 Oct 2021
Historique:
pubmed: 25 9 2021
medline: 27 10 2021
entrez: 24 9 2021
Statut: epublish

Résumé

This report presents a novel approach for detecting and visualizing small to trace amounts of DNA in a sample. By utilizing both the change in emission spectrum and change in fluorescence lifetime, there is a significant increase in detection sensitivity allowing for the imaging/visualizing of a picograms amount of DNA in a microliters volume. As in the previous reports, one of the oldest DNA intercalators, Ethidium Bromide (EtBr), is employed as a model system. With this new approach, it is feasible to visualize just a few hundred picograms of DNA without the need for prior DNA amplification. The sensitivity can later be largely improved by using an intercalator that exhibits a higher affinity to DNA and a larger fluorescence change upon binding to DNA (

Identifiants

DOI: 10.1039/d1an01391b PMID: 34559174
pubmed: 34559174
doi: 10.1039/d1an01391b
doi:

Substances chimiques

Intercalating Agents 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

6520-6527

Auteurs

Luca Ceresa (L)

Department of Physics and Astronomy, Texas Christian University, 2800 S. University, Dr. Fort Worth, Texas, 76129, USA. luca.ceresa@tcu.edu.
ORCID: 0000-0001-8771-496X

Emma Kitchner (E)

Department of Physics and Astronomy, Texas Christian University, 2800 S. University, Dr. Fort Worth, Texas, 76129, USA. luca.ceresa@tcu.edu.
ORCID: 0000-0002-9226-2432

Michael Seung (M)

Department of Physics and Astronomy, Texas Christian University, 2800 S. University, Dr. Fort Worth, Texas, 76129, USA. luca.ceresa@tcu.edu.

Magdalena M Bus (MM)

Center for Human Identification, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, CBH-250, Ft Worth, TX 76107, USA.
Department of Microbiology, Immunology and Genetics, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Ft Worth, TX 76107, USA.

Bruce Budowle (B)

Center for Human Identification, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, CBH-250, Ft Worth, TX 76107, USA.
Department of Microbiology, Immunology and Genetics, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Ft Worth, TX 76107, USA.

Jose Chavez (J)

Department of Physics and Astronomy, Texas Christian University, 2800 S. University, Dr. Fort Worth, Texas, 76129, USA. luca.ceresa@tcu.edu.
ORCID: 0000-0002-3834-3573

Ignacy Gryczynski (I)

Department of Physics and Astronomy, Texas Christian University, 2800 S. University, Dr. Fort Worth, Texas, 76129, USA. luca.ceresa@tcu.edu.

Zygmunt Gryczynski (Z)

Department of Physics and Astronomy, Texas Christian University, 2800 S. University, Dr. Fort Worth, Texas, 76129, USA. luca.ceresa@tcu.edu.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › A novel approach to imaging and visualization...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Intercalants › A novel approach to imaging and visualization...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › A novel approach to imaging and visualization...