Reduced frequency of perforin-positive CD8+ T cells in menstrual effluent of endometriosis patients.
Adult
B-Lymphocyte Subsets
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Cytokines
/ metabolism
Cytotoxicity, Immunologic
Endometriosis
/ immunology
Female
Humans
Intercellular Adhesion Molecule-1
/ blood
Killer Cells, Natural
/ immunology
Lymphocyte Count
Menstrual Cycle
/ metabolism
Perforin
/ metabolism
T-Lymphocytes
/ immunology
T-Lymphocytes, Regulatory
/ immunology
Endometriosis
Menstrual effluent
Perforin
T cells
Journal
Journal of reproductive immunology
ISSN: 1872-7603
Titre abrégé: J Reprod Immunol
Pays: Ireland
ID NLM: 8001906
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
28
03
2021
revised:
20
08
2021
accepted:
15
09
2021
pubmed:
27
9
2021
medline:
29
3
2022
entrez:
26
9
2021
Statut:
ppublish
Résumé
Endometriosis is a widespread disease and commonly reduces the life quality of those affected. Scientific literature indicates different underlying immunological changes. Frequently examined tissues are peripheral blood, endometrial tissue and peritoneal fluid. Yet, knowledge on immunological differences in menstrual effluent (ME) is scarce. In this study, between January 2018 and August 2019, 12 women with endometriosis (rASRM classification: stages I-IV) and 11 healthy controls were included. ME was collected using menstrual cups and venous blood samples (PB) were taken. Mononuclear cells were obtained from ME (MMC) and PB (PBMC) and analyzed using flow cytometry. Concentrations of cell adhesion molecules (ICAM-I and VCAM-I) and cytokines (IL-6, IL-8 and TNF-α) were measured using ELISA. CD8 + T cells obtained from ME were significantly less often perforin-positive in women with endometriosis compared to healthy controls. A comparison between MMC and PBMC revealed that MMC contained significantly less T cells and more B cells. The CD4/CD8 ratio was significantly higher in MMC, and Tregs were significantly less frequently in MMC. In ME, T cells and NK cells expressed significantly more CD69. NK cells obtained from ME were predominantly CD56
Identifiants
pubmed: 34563756
pii: S0165-0378(21)00154-6
doi: 10.1016/j.jri.2021.103424
pii:
doi:
Substances chimiques
Cytokines
0
Perforin
126465-35-8
Intercellular Adhesion Molecule-1
126547-89-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103424Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.