Characterization of antibody-antigen interactions using biolayer interferometry.

This protocol describes the use of a biolayer interferometry platform for assessing antibody-antigen interactions. The protocol focuses on affinity determination and epitope binning, although the system can be utilized for measuring any protein-protein interaction. Readings are collected in real time, allowing the use of unlabeled molecules, and data can thus be obtained in a fast and easy manner. Experiments should be carefully designed, taking into consideration the tested interaction, available sensors, and suitable controls. For complete details on the use and execution of this protocol, please refer to Noy-Porat et al. (2021).

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

Patent application for the described antibodies in Noy-Porat et al. (2021) was filed by the Israel Institute for Biological Research. None of the authors declared any additional competing interests.