Effects of a BMI1008 mixture on postoperative pain in a rat model of incisional pain.
Analgesia
/ methods
Analgesics
/ administration & dosage
Animals
Behavior, Animal
Dexamethasone
/ administration & dosage
Dose-Response Relationship, Drug
Lidocaine
/ administration & dosage
Male
Methylene Blue
/ administration & dosage
Pain Management
/ methods
Pain, Postoperative
/ prevention & control
Rats
Rats, Sprague-Dawley
Surgical Wound
Vitamin B Complex
/ administration & dosage
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
23
04
2021
accepted:
31
08
2021
entrez:
27
9
2021
pubmed:
28
9
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The purpose of this study was to evaluate the analgesic effect of BMI1008 (a new drug containing lidocaine, methylene blue, dexamethasone and vitamin B complex) and to investigate the analgesic effect of lidocaine and BMI-L (other components of BMI1008 except lidocaine) at different concentrations in a rat model of incisional pain. Male Sprague-Dawley rats (250-300 g) were used for the incisional pain model simulating postoperative pain. After the operation, normal saline, various concentrations of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine were injected at the incision site. The preventive analgesic effect was evaluated using BMI1008 administered 30 min before and immediately after the operation. In addition, BMI1008 was compared with positive controls using intraperitoneal ketorolac 30 mg/kg and fentanyl 0.5 μg/kg. The mechanical withdrawal threshold was measured with a von Frey filament. The analgesic effect according to the concentration of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine showed a concentration-dependent response and statistically significant difference among the groups (P <0.001, P <0.001, and P <0.001, respectively). The analgesic effect according to the time point of administration (before and after the operation) showed no evidence of a statistically significant difference between the groups (P = 0.170). Compared with the positive control groups, the results showed a statistically significant difference between the groups (P = 0.024). BMI1008 showed its analgesic effect in a rat model of incisional pain in a concentration-dependent manner. Moreover, BMI-L showed an additive effect on the analgesic effect of lidocaine.
Sections du résumé
BACKGROUND
The purpose of this study was to evaluate the analgesic effect of BMI1008 (a new drug containing lidocaine, methylene blue, dexamethasone and vitamin B complex) and to investigate the analgesic effect of lidocaine and BMI-L (other components of BMI1008 except lidocaine) at different concentrations in a rat model of incisional pain.
METHODS
Male Sprague-Dawley rats (250-300 g) were used for the incisional pain model simulating postoperative pain. After the operation, normal saline, various concentrations of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine were injected at the incision site. The preventive analgesic effect was evaluated using BMI1008 administered 30 min before and immediately after the operation. In addition, BMI1008 was compared with positive controls using intraperitoneal ketorolac 30 mg/kg and fentanyl 0.5 μg/kg. The mechanical withdrawal threshold was measured with a von Frey filament.
RESULTS
The analgesic effect according to the concentration of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine showed a concentration-dependent response and statistically significant difference among the groups (P <0.001, P <0.001, and P <0.001, respectively). The analgesic effect according to the time point of administration (before and after the operation) showed no evidence of a statistically significant difference between the groups (P = 0.170). Compared with the positive control groups, the results showed a statistically significant difference between the groups (P = 0.024).
CONCLUSION
BMI1008 showed its analgesic effect in a rat model of incisional pain in a concentration-dependent manner. Moreover, BMI-L showed an additive effect on the analgesic effect of lidocaine.
Identifiants
pubmed: 34570780
doi: 10.1371/journal.pone.0257267
pii: PONE-D-21-13470
pmc: PMC8476004
doi:
Substances chimiques
Analgesics
0
Vitamin B Complex
12001-76-2
Dexamethasone
7S5I7G3JQL
Lidocaine
98PI200987
Methylene Blue
T42P99266K
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0257267Déclaration de conflit d'intérêts
BMI Korea supported BMI 1008 materials and research grants for this research. BMI Korea was only related to providing the products in development, but not related to employment, conultancy, patents and marketed products. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology [Grant No. NRF-2020R1C1C1011263]. BMI Korea and NRF had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. This did not alter our adherence to PLOS ONE policies on sharing data and materials.
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