Use of real-time PCR as an alternative to conventional genotyping methods for the laboratory detection of lymphogranuloma venereum (LGV).
Bacterial Outer Membrane Proteins
/ genetics
Chlamydia trachomatis
/ classification
DNA, Bacterial
/ genetics
Genome, Bacterial
/ genetics
Genotype
Humans
Lymphogranuloma Venereum
/ diagnosis
Molecular Diagnostic Techniques
/ methods
Real-Time Polymerase Chain Reaction
/ methods
Rectum
/ microbiology
Reproducibility of Results
Sensitivity and Specificity
Sequence Analysis, DNA
Serogroup
Chlamydia trachomatis
Lymphogranuloma venereum
Outer membrane protein A (ompA)
Real-time PCR
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
21
06
2021
revised:
20
08
2021
accepted:
21
08
2021
pubmed:
28
9
2021
medline:
1
2
2022
entrez:
27
9
2021
Statut:
ppublish
Résumé
Lymphogranuloma venereum (LGV) can be differentiated from non-LGV chlamydial infection using Sanger sequencing or molecular assays, including those that are commercially-available internationally. Here, we describe the performance of a rapid real-time PCR (RT-PCR)-based strategy in differentiating Chlamydia trachomatis infections associated with LGV or non-LGV serovars. One hundred three rectal swabs, previously genotyped using Sanger sequencing of the ompA gene as a reference method, were tested in the RT-PCR assays. All non-LGV specimens were correctly identified, but the RT-PCR failed to detect 1 LGV specimen, resulting in a sensitivity of 87.5% for the non-LGV/LGV RT-PCR assay. Additional performance characteristics (e.g., specificity, accuracy, and reproducibility) were all between 93% and 100% with a limit of detection ≤100 copies/reaction. Thus, this rapid RT-PCR method for LGV detection in clinical specimens is comparable to the reference method.
Identifiants
pubmed: 34571353
pii: S0732-8893(21)00224-8
doi: 10.1016/j.diagmicrobio.2021.115532
pmc: PMC8650105
mid: NIHMS1747714
pii:
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
DNA, Bacterial
0
OMPA outer membrane proteins
149024-69-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115532Subventions
Organisme : Intramural CDC HHS
ID : CC999999
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors report no conflicts of interest relevant to this article.
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