Prevalence and Molecular Characterization of Human Bocavirus Detected in Croatian Children with Respiratory Infection.
Adolescent
Child
Child, Preschool
Coinfection
/ diagnosis
Croatia
/ epidemiology
Feces
/ virology
Female
Genotype
High-Throughput Nucleotide Sequencing
Human bocavirus
/ genetics
Humans
Infant
Infant, Newborn
Male
Parvoviridae Infections
/ diagnosis
Phylogeny
Prevalence
Respiratory Tract Infections
/ diagnosis
Sequence Analysis, DNA
Croatia
bocavirus
phylogeny
prevalence
recombination
respiratory tract
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
31 08 2021
31 08 2021
Historique:
received:
19
07
2021
revised:
24
08
2021
accepted:
26
08
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
15
2
2022
Statut:
epublish
Résumé
Human bocavirus (HBoV) 1 is considered an important respiratory pathogen, while the role of HBoV2-4 in clinical disease remains somewhat controversial. Since, they are characterized by a rapid evolution, worldwide surveillance of HBoVs' genetics is necessary. This study explored the prevalence of HBoV genotypes in pediatric patients with respiratory tract infection in Croatia and studied their phylogeny. Using multiplex PCR for 15 respiratory viruses, we investigated 957 respiratory samples of children up to 18 years of age with respiratory tract infection obtained from May 2017 to March 2021 at two different hospitals in Croatia. Amplification of HBoV near-complete genome or three overlapping fragments was performed, sequenced, and their phylogenetic inferences constructed. HBoV was detected in 7.6% children with a median age of 1.36 years. Co-infection was observed in 82.2% samples. Sequencing was successfully performed on 29 HBoV positive samples, and all belonged to HBoV1. Croatian HBoV1 sequences are closely related to strains isolated worldwide, and no phylogenetic grouping based on mono- or co-infection cases or year of isolation was observed. Calculated rates of evolution for HBoV1 were 10
Identifiants
pubmed: 34578309
pii: v13091728
doi: 10.3390/v13091728
pmc: PMC8473146
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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