Comparative Analysis of Novel Strains of Porcine Astrovirus Type 3 in the USA.
Animals
Antigens, Viral
Astroviridae Infections
/ veterinary
Encephalitis, Viral
/ veterinary
Epitopes
Genome, Viral
Mamastrovirus
/ genetics
Nucleic Acid Conformation
Open Reading Frames
Phylogeny
RNA, Viral
/ chemistry
Swine
Swine Diseases
/ virology
Untranslated Regions
Viral Proteins
/ chemistry
VPg: UTR
mamastrovirus 22
polioencephalomyelitis
porcine astrovirus type 3
pseudoknot
untranslated region
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
received:
11
08
2021
revised:
12
09
2021
accepted:
14
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
9
2
2022
Statut:
epublish
Résumé
Porcine astrovirus type 3 (PoAstV3) has been previously identified as a cause of polioencephalomyelitis in swine and continues to cause disease in the US swine industry. Herein, we describe the characterization of both untranslated regions, frameshifting signal, putative genome-linked virus protein (VPg) and conserved antigenic epitopes of several novel PoAstV3 genomes. Twenty complete coding sequences (CDS) were obtained from 32 diagnostic cases originating from 11 individual farms/systems sharing a nucleotide (amino acid) percent identity of 89.74-100% (94.79-100%), 91.9-100% (96.3-100%) and 90.71-100% (93.51-100%) for ORF1a, ORF1ab and ORF2, respectively. Our results indicate that the 5'UTR of PoAstV3 is highly conserved highlighting the importance of this region in translation initiation while their 3'UTR is moderately conserved among strains, presenting alternative configurations including multiple putative protein binding sites and pseudoknots. Moreover, two predicted conserved antigenic epitopes were identified matching the 3' termini of VP27 of PoAstV3 USA strains. These epitopes may aid in the design and development of vaccine components and diagnostic assays useful to control outbreaks of PoAstV3-associated CNS disease. In conclusion, this is the first analysis predicting the structure of important regulatory motifs of neurotropic mamastroviruses, which differ from those previously described in human astroviruses.
Identifiants
pubmed: 34578440
pii: v13091859
doi: 10.3390/v13091859
pmc: PMC8472076
pii:
doi:
Substances chimiques
Antigens, Viral
0
Epitopes
0
RNA, Viral
0
Untranslated Regions
0
Viral Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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