Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation.
PET hydrolase
biocatalysis
chemical recycling
enzymes
polymers
Journal
ChemSusChem
ISSN: 1864-564X
Titre abrégé: ChemSusChem
Pays: Germany
ID NLM: 101319536
Informations de publication
Date de publication:
10 Jan 2022
10 Jan 2022
Historique:
revised:
28
09
2021
received:
09
09
2021
pubmed:
30
9
2021
medline:
15
1
2022
entrez:
29
9
2021
Statut:
ppublish
Résumé
There is keen interest to develop new technologies to recycle the plastic poly(ethylene terephthalate) (PET). To this end, the use of PET-hydrolyzing enzymes has shown promise for PET deconstruction to its monomers, terephthalate (TPA) and ethylene glycol (EG). Here, the Ideonella sakaiensis PETase wild-type enzyme was compared to a previously reported improved variant (W159H/S238F). The thermostability of each enzyme was compared and a 1.45 Å resolution structure of the mutant was described, highlighting changes in the substrate binding cleft compared to the wild-type enzyme. Subsequently, the performance of the wild-type and variant enzyme was compared as a function of temperature, substrate morphology, and reaction mixture composition. These studies showed that reaction temperature had the strongest influence on performance between the two enzymes. It was also shown that both enzymes achieved higher levels of PET conversion for substrates with moderate crystallinity relative to amorphous substrates. Finally, the impact of product accumulation on reaction progress was assessed for the hydrolysis of both PET and bis(2-hydroxyethyl) terephthalate (BHET). Each enzyme displayed different inhibition profiles to mono(2-hydroxyethyl) terephthalate (MHET) and TPA, while both were sensitive to inhibition by EG. Overall, this study highlights the importance of reaction conditions, substrate selection, and product accumulation for catalytic performance of PET-hydrolyzing enzymes, which have implications for enzyme screening in the development of enzyme-based polyester recycling.
Identifiants
pubmed: 34587366
doi: 10.1002/cssc.202101932
doi:
Substances chimiques
Plastics
0
Polyethylene Terephthalates
0
Hydrolases
EC 3.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202101932Subventions
Organisme : Alliance for Sustainable Energy, LLC
Organisme : National Renewable Energy Laboratory (NREL)
Organisme : U.S. Department of Energy (DOE)
ID : DE-AC36-08GO28308
Organisme : U.S. DOE, Office of Energy Efficiency and Renewable Energy
Organisme : Advanced Manufacturing Office (AMO)
Organisme : Bioenergy Technologies Office (BETO)
ID : DE-AC36-08GO28308
Organisme : University of Portsmouth
ID : DE-AC36-08GO28308
Organisme : Research England
Organisme : U.S. Government
Organisme : NREL Laboratory Directed Research and Development
Organisme : Research England
ID : BB/P011918/1
Organisme : University of Portsmouth Faculty of Science
Organisme : Diamond Light Source
ID : MX-23269
Organisme : Diamond Light Source
ID : I03
Informations de copyright
© 2021 Wiley-VCH GmbH.
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