Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA.

In Situ Hybridization, Fluorescence / methods RNA, Viral / isolation & purification Viruses / genetics

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
01 10 2021
Historique:
received: 12 05 2021
accepted: 09 09 2021
entrez: 2 10 2021
pubmed: 3 10 2021
medline: 14 10 2021
Statut: epublish

Résumé

The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.

Identifiants

DOI: 10.1038/s41598-021-98972-z PMID: 34599242 PMC: PMC8486776
pubmed: 34599242
doi: 10.1038/s41598-021-98972-z
pii: 10.1038/s41598-021-98972-z
pmc: PMC8486776
doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

19579

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/V001868/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 110164/Z/15/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N010744/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom

Informations de copyright

© 2021. The Author(s).

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Auteurs

Christof Hepp (C)

Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK. christof.hepp@physics.ox.ac.uk.

Nicolas Shiaelis (N)

Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK.

Nicole C Robb (NC)

Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK.
Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.

Alison Vaughan (A)

Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.

Philippa C Matthews (PC)

Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.

Nicole Stoesser (N)

Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.
NIHR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance at the University of Oxford in Partnership With Public Health England, University of Oxford, Oxford, UK.

Derrick Crook (D)

Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.
NIHR Biomedical Research Centre, University of Oxford, Oxford, UK.
NIHR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance at the University of Oxford in Partnership With Public Health England, University of Oxford, Oxford, UK.

Achillefs N Kapanidis (AN)

Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, UK. kapanidis@physics.ox.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Hybridation in situ › Hybridation fluorescente in situ › Viral detection and identification in 20 min...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Viral detection and identification in 20 min...
questionsmedicales.fr › Virus › Viral detection and identification in 20 min...