Development of an in vitro neuroblastoma 3D model and its application for sterigmatocystin-induced cytotoxicity testing.
Blotting, Western
Cell Culture Techniques, Three Dimensional
/ methods
Cell Line, Tumor
/ cytology
Cell Movement
/ drug effects
Comet Assay
/ methods
Fluorescent Antibody Technique
Humans
Mycotoxins
/ toxicity
Neuroblastoma
Reactive Oxygen Species
/ metabolism
Spheroids, Cellular
/ drug effects
Sterigmatocystin
/ toxicity
Toxicity Tests
/ methods
3D spheroid
Cytotoxicity
Mechanisms of action
Neuroblastoma
Sterigmatocystin
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
ISSN: 1873-6351
Titre abrégé: Food Chem Toxicol
Pays: England
ID NLM: 8207483
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
04
07
2021
revised:
04
10
2021
accepted:
07
10
2021
pubmed:
12
10
2021
medline:
10
2
2022
entrez:
11
10
2021
Statut:
ppublish
Résumé
Given the increasing importance of establishing better risk assessments for mycotoxins, novel in vitro tools for the evaluation of their toxicity are mandatory. In this study, an in vitro 3D spheroid model from SH-SY5Y cells, a human neuroblastoma cell line, was developed, optimized and characterized to test the cytotoxic effects caused by the mycotoxin sterigmatocystin (STE). STE induced a concentration- and time-dependent cell viability decrease in spheroids. Spheroids displayed cell disaggregation after STE exposure, increasing in a dose-dependent manner and over time. STE also induced apoptosis as confirmed by immunofluorescence staining and Western blot. Following the decreased proliferation and increased apoptosis, STE cytostasis effects were observed by migration assays both in 2D and 3D cell culture. Increased ROS generation, as well as DNA damage were also observed. Taken together, these data highlight the cytotoxic properties of STE and suggest that cell culture models play a pivotal role in the toxicological risk assessment of mycotoxins. The evaluation of cytotoxicity in spheroids (3D) rather than monolayer cultures (2D) is expected to more accurately reflect in vivo-like cell behaviour.
Identifiants
pubmed: 34634377
pii: S0278-6915(21)00638-4
doi: 10.1016/j.fct.2021.112605
pii:
doi:
Substances chimiques
Mycotoxins
0
Reactive Oxygen Species
0
Sterigmatocystin
10048-13-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112605Informations de copyright
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