In vitro genotoxicity of dibutyl phthalate on A549 lung cells at air-liquid interface in exposure concentrations relevant at workplaces.
A549 Cells
Adult
Air
Air Pollutants, Occupational
/ toxicity
Cell Survival
/ drug effects
Chromosomal Instability
/ drug effects
Comet Assay
DNA Damage
Dibutyl Phthalate
/ toxicity
Humans
Inhalation Exposure
Male
Malondialdehyde
/ metabolism
Micronucleus Tests
Models, Biological
Mutagens
/ toxicity
Occupational Exposure
Oxidative Stress
/ drug effects
Plasticizers
/ toxicity
Pulmonary Alveoli
/ metabolism
Workplace
air-liquid interface
alveolar deposition
dibutyl phthalate
genotoxicity
oxidative stress
Journal
Environmental and molecular mutagenesis
ISSN: 1098-2280
Titre abrégé: Environ Mol Mutagen
Pays: United States
ID NLM: 8800109
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
14
09
2021
received:
19
07
2021
accepted:
07
10
2021
pubmed:
13
10
2021
medline:
18
12
2021
entrez:
12
10
2021
Statut:
ppublish
Résumé
The ubiquitous use of phthalates in various materials and the knowledge about their potential adverse effects is of great concern for human health. Several studies have uncovered their role in carcinogenic events and suggest various phthalate-associated adverse health effects that include pulmonary diseases. However, only limited information on pulmonary toxicity is available considering inhalation of phthalates as the route of exposure. While in vitro studies are often based on submerged exposures, this study aimed to expose A549 alveolar epithelial cells at the air-liquid interface (ALI) to unravel the genotoxic and oxidative stress-inducing potential of dibutyl phthalate (DBP) with concentrations relevant at occupational settings. Within this scope, a computer modeling approach calculating alveolar deposition of DBP particles in the human lung was used to define in vitro ALI exposure conditions comparable to potential occupational DBP exposures. The deposited mass of DBP ranged from 0.03 to 20 ng/cm
Substances chimiques
Air Pollutants, Occupational
0
Mutagens
0
Plasticizers
0
Dibutyl Phthalate
2286E5R2KE
Malondialdehyde
4Y8F71G49Q
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
490-501Informations de copyright
© 2021 The Authors. Environmental and Molecular Mutagenesis published by Wiley Periodicals LLC on behalf of Environmental Mutagen Society.
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