Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae.
Pmk1 pathway
actin ring
appressorium formation
autophagy
cAMP pathway
endocytosis
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
24
09
2021
received:
24
06
2021
accepted:
24
09
2021
pubmed:
13
10
2021
medline:
3
2
2022
entrez:
12
10
2021
Statut:
ppublish
Résumé
Endocytosis plays key roles during infection of plant-pathogenic fungi, but its regulatory mechanisms are still largely unknown. Here, we identified a putative endocytosis-related gene, PAL1, which was highly expressed in appressorium of Magnaporthe oryzae, and was found to be important for appressorium formation and maturation. Deletion of PAL1 significantly reduced the virulence of M. oryzae due to defects in appressorial penetration and invasive growth in host cells. The Pal1 protein interacted and colocalized with the endocytosis protein Sla1, suggesting it is involved in endocytosis. The Δpal1 mutant was significantly reduced in appressorium formation, which was recovered by adding exogenous cAMP and 3-isobutyl-1-methylxanthine (IBMX). Moreover, the phosphorylation level of Pmk1 in Δpal1 was also reduced, suggesting Pal1 functions upstream of both the cAMP and Pmk1 signalling pathways. As a consequence, the utilization of glycogen and lipid, appressorial autophagy, actin ring formation, localization of septin proteins, as well as turgor accumulation were all affected in the Δpal1 mutant. Taken together, Pal1 regulates cAMP and the Pmk1 signalling pathway for appressorium formation and maturation to facilitate infection of M. oryzae.
Identifiants
pubmed: 34636149
doi: 10.1111/mpp.13149
pmc: PMC8659611
doi:
Substances chimiques
Fungal Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
133-147Informations de copyright
© 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
Références
Microsc Res Tech. 2000 Dec 15;51(6):547-62
pubmed: 11169857
PLoS Genet. 2017 May 25;13(5):e1006814
pubmed: 28542408
Mol Plant Microbe Interact. 2013 Dec;26(12):1407-16
pubmed: 23902261
Environ Microbiol. 2015 Aug;17(8):2969-81
pubmed: 25808678
PLoS Pathog. 2011 Jan 20;7(1):e1001261
pubmed: 21283781
Annu Rev Phytopathol. 2007;45:437-56
pubmed: 17489691
Plant Cell. 2000 Sep;12(9):1703-18
pubmed: 11006342
Fungal Genet Biol. 2005 Dec;42(12):963-75
pubmed: 16291501
Plant Cell. 1998 Aug;10(8):1361-74
pubmed: 9707535
Environ Microbiol. 2017 Mar;19(3):1008-1016
pubmed: 28165657
Mol Plant Pathol. 2022 Jan;23(1):133-147
pubmed: 34636149
Plant Cell. 2005 Apr;17(4):1317-29
pubmed: 15749760
Plant Cell. 1999 Oct;11(10):2013-30
pubmed: 10521529
Plant Cell. 1995 Nov;7(11):1869-78
pubmed: 8535140
Science. 2006 Apr 28;312(5773):580-3
pubmed: 16645096
J Cell Sci. 2006 Nov 15;119(Pt 22):4585-7
pubmed: 17093262
Sci Signal. 2021 May 25;14(684):
pubmed: 34035143
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15967-72
pubmed: 19717456
Mol Biol Cell. 2005 Sep;16(9):4124-38
pubmed: 15975911
Methods Mol Biol. 2012;835:255-69
pubmed: 22183659
Dev Cell. 2006 Jul;11(1):33-46
pubmed: 16824951
Genes Dev. 1996 Nov 1;10(21):2696-706
pubmed: 8946911
Cold Spring Harb Perspect Biol. 2012 Dec 01;4(12):
pubmed: 23209152
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3179-84
pubmed: 23382235
New Phytol. 2018 Aug;219(3):1031-1047
pubmed: 29663402
J Cell Biol. 2001 Jan 8;152(1):51-64
pubmed: 11149920
Mol Plant Microbe Interact. 2010 Mar;23(3):317-31
pubmed: 20121453
Mol Plant Pathol. 2017 Feb;18(2):222-237
pubmed: 26950649
Environ Microbiol. 2020 Dec;22(12):5373-5386
pubmed: 33000483
Mol Biol Cell. 2004 Mar;15(3):1101-11
pubmed: 14699058
Mol Biol Cell. 2012 Feb;23(4):657-68
pubmed: 22190733
Mol Plant Pathol. 2009 May;10(3):337-46
pubmed: 19400837
Mol Plant Pathol. 2012 May;13(4):414-30
pubmed: 22471698
Cell. 2005 Oct 21;123(2):305-20
pubmed: 16239147
Nat Rev Microbiol. 2009 Mar;7(3):185-95
pubmed: 19219052
Mol Cells. 1998 Dec 31;8(6):698-704
pubmed: 9895122
Environ Microbiol. 2020 Dec;22(12):5414-5432
pubmed: 33073491
PLoS Genet. 2017 Jan 10;13(1):e1006557
pubmed: 28072818
Science. 2012 Jun 22;336(6088):1590-5
pubmed: 22723425
Dev Cell. 2005 Jul;9(1):87-98
pubmed: 15992543
PLoS Pathog. 2015 Apr 22;11(4):e1004851
pubmed: 25901357
PLoS Pathog. 2017 Jun 19;13(6):e1006449
pubmed: 28628655
Annu Rev Microbiol. 2003;57:177-202
pubmed: 14527276