Genomic diversities of ctxB, tcpA and rstR alleles of Vibrio cholerae O139 strains isolated from Odisha, India.
Journal
Environmental microbiology reports
ISSN: 1758-2229
Titre abrégé: Environ Microbiol Rep
Pays: United States
ID NLM: 101499207
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
30
07
2021
accepted:
05
10
2021
pubmed:
21
10
2021
medline:
25
5
2022
entrez:
20
10
2021
Statut:
ppublish
Résumé
The genome of Vibrio cholerae O139 strains has undergone cryptic changes since its first emergence in 1992 in South India. This study aimed to determine the presence of genotypic changes marked in ctxB, tcpA and rstR genes located within the CTX prophages among the strains of V. cholerae O139 isolated from 1999 to 2017 in Odisha. Antibiotic susceptibility test was conducted on 59 V. cholerae O139 strains. A conventional PCR assay was done for ctxB gene typing followed by sequencing along with identification of rstR and tcpA gene. Pulsed-field gel electrophoresis (PFGE) was carried out to reveal clonal variations among the V. cholerae O139 strains. Among V. cholerae O139 isolates more than 60% showed resistance to ampicillin, co-trimoxazole, furazolidone, streptomycin, neomycin and nalidixic acid. The ctxB sequencing and rstR allele-specific PCR assay revealed the presence of three genotypes 1, 3 and 4 with at least one copy of CTX Calc φ in addition to CTX ET and CTX Cl prophages in V. cholerae O139 isolates. PFGE analysis revealed 13 pulsotypes with two clades having 60% similarity among V. cholerae O139 strains. The circulating V. cholerae O139 strains in Odisha showed variation in genotypes with multiple clonal expansions over the years.
Identifiants
pubmed: 34668341
doi: 10.1111/1758-2229.13016
doi:
Substances chimiques
Cholera Toxin
9012-63-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
376-384Informations de copyright
© 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.
Références
Baker-Austin, C., McArthur, J.V., Lindell, A.H., Wright, M.S., Tuckfield, R.C., Gooch, J., et al. (2009) Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus. Micro Ecol 57: 151-159.
Bauer, A.W., Kirby, W.M., Sherris, J.C., and Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493-496.
Chatterjee, S., Ghosh, K., Raychoudhuri, A., Pan, A., Bhattacharya, M.K., Mukhopadhyay, A.K., et al. (2007) Phenotypic and genotypic traits and epidemiological implication of Vibrio cholerae O1 and O139 strains in India during 2003. J Med Microbiol 56: 824-832.
Cooper, K.L., Luey, C.K., Bird, M., Terajima, J., Nair, G.B., Kam, K.M., et al. (2006) Development and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio cholerae. Food Patho Dis 3: 51-58.
Davis, B.M., Kimsey, H.H., Chang, W., and Waldor, M.K. (1999) The Vibrio cholerae O139 Calcutta bacteriophage CTXφ is infectious and encodes a novel repressor. J Bacteriol 181: 6779-6787.
Faruque, S.M., Albert, M.J., and Mekalanos, J.J. (1998a) Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol Mol Bio Rev 62: 1301-1314.
Faruque, S.M., Alim, A.R.A., Roy, S.K., Khan, F., Nair, G.B., Sack, R.B., and Albert, M.J. (1994) Molecular analysis of rRNA and cholera toxin genes carried by the new epidemic strain of toxigenic Vibrio cholerae O139 synonym Bengal. J Clin Microbiol 32: 1050-1053.
Faruque, S.M., Sack, D.A., Sack, R.B., Colwell, R.R., Takeda, Y., and Nair, G.B. (2003) Emergence and evolution of Vibrio cholerae O139. Proc Natl Acad Sci 100: 1304-1309.
Faruque, S.M., Saha, M.N., Alim, A.A., Albert, M.J., Islam, K.N., Islam, K.M., and Mekalanos, J.J. (1998b) Analysis of clinical and environmental strains of nontoxigenic Vibrio cholerae for susceptibility to CTXΦ: molecular basis for origination of new strains with epidemic potential. Infect Immun 66: 5819-5825.
Faruque, S.M., Saha, M.N., Asadulghani, P.K., Bhadra, P.K., and Bhattacharya, S.K. (2000) Genomic diversity among Vibrio cholerae O139 strains isolated in Bangladesh and India between 1992 and 1998. FEMS Microbiol Lett 184: 279-284.
Ghosh, P., Naha, A., Pazhani, G.P., Ramamurthy, T., and Mukhopadhyay, A.K. (2014) Genetic traits of Vibrio cholerae O1 Haitian isolates that are absent in contemporary strains from Kolkata, India. PLoS One 9: e112973.
Ghosh, R., Sharma, N.C., Halder, K., Bhadra, R.K., Chowdhury, G., Pazhani, G.P., et al. (2016) Phenotypic and genetic heterogeneity in Vibrio cholerae O139 isolated from cholera cases in Delhi, India during 2001-2006. Front Microbiol 9: 1250.
Glass, R.I., Huq, I., Alim, A.R., and Yunus, M. (1980) Emergence of multiply antibiotic-resistant Vibrio cholerae in Bangladesh. J Infect Dis 142: 939-942.
Kaper, J., Morris, J.G.J., and Levine, M. (1995) Cholera. Clin Microbiol Rev 8: 48-86.
Kimsey, H.H., and Waldor, M.K. (1998) CTXφ immunity: application in the development of cholera vaccines. Proc Natl Acad Sci 95: 7035-7039.
Kumar, P., Jain, M., Goel, A.K., Bhadauria, S., Sharma, S.K., Kamboj, D.V., et al. (2009) A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India. J Med Microbiol 58: 234-238.
Li, B.S., Xiao, Y., Wang, D.C., Tan, H.L., Ke, B.X., He, D.M., et al. (2016) Genetic relatedness of selected clinical Vibrio cholerae O139 isolates from the southern coastal area of China over a 20-year period. Epidemio Infect 144: 2679-2687.
Lin, W., Fullner, K.J., Clayton, R., Sexton, J.A., Rogers, M.B., Calia, K.E., et al. (1999) Identification of a Vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage. Proc Natl Acad Sci 96: 1071-1076.
Mazel, D., Dychinco, B., Webb, V.A., and Davies, J. (1998) A distinctive class of integron in the Vibrio cholerae genome. Science 280: 605-608.
Mitra, R.K., Nandy, R.K., Ramamurthy, T., Bhattacharya, S.K., Yamasaki, S., Shimada, T., et al. (2001) Molecular characterization of rough variants of Vibrio cholerae isolated from hospitalised patients with diarrhoea. J Med Microbiol 50: 268-276.
Morita, M., Ohnishi, M., Arakawa, E., Bhuiyan, N.A., Nusrin, S., Alam, M., et al. (2008) Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor. Microbio Immunol 52: 314-317.
Mukhopadhyay, A.K., Basu, A., Garg, P., Bag, P.K., Ghosh, A., Bhttacharya, S.K., et al. (1998) Molecular epidemiology of reemergent Vibrio cholerae O139 Bengal in India. J Clini Microbiol 36: 2149-2152.
Mukhopadhyay, A.K., Garg, S., Mitra, R., Basu, A., Rajendran, K., Dutta, D., et al. (1996) Temporal shifts in traits of Vibrio cholerae strains isolated from hospitalized patients in Calcutta: a 3-year (1993 to 1995) analysis. J Clin Microbiol 34: 2537-2543.
Nair, G.B., Misra, S., Bhadra, R.K., and Pal, S.C. (1987) Evaluation of the multitest medium for rapid presumptive identification of Vibrio cholerae from environmental sources. Appl Environ Microbiol 53: 1203-1205.
Nair, G.B., Ramamurthy, T., Bhattacharya, S.K., Mukhopadhyay, A.K., Garg, S., Bhattacharya, M.K., et al. (1994) Spread of Vibrio cholerae 0139 Bengal in India. J Infect Dis 169: 1029-1034.
Nusrin, S., Khan, G.Y., Bhuiyan, N.A., Ansaruzzaman, M., Hossain, M.A., Safa, A., et al. (2004) Diverse CTX phages among toxigenic Vibrio cholerae O1 and O139 strains isolated between 1994 and 2002 in an area where cholera is endemic in Bangladesh. J Clin Microbiol 42: 5854-5856.
Pal, B.B., Khuntia, H.K., Nayak, S.R., Mohanty, A., and Biswal, B. (2017) Vibrio cholerae O1 Ogawa strains carrying ctxB7 allele caused a large cholera outbreak during 2014 in the tribal areas of Odisha, India. Jpn J Infect Dis 70: 549-553.
Pal, B.B., Mohanty, A., Biswas, B., and Nayak, S.R. (2019) New variant of Vibrio cholerae O139 in Odisha. Ind J Clin Microbiol 57: e01877-e01818.
Politzer, R. (1959) Cholera. Monograph series, no. 43. Geneva, Switzerland: World Health Organization.
Ramachandran, D., Bhanumathi, R., and Singh, D.V. (2007) Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae. J Med Microbiol 56: 346-351.
Ramamurthy, T.P., Bag, P.K., Pal, A., Bhattacharya, S.K., Bhattacharya, M.K., and Shimada, T. (1993) Virulence patterns of Vibrio cholerae non-01 strains isolated from hospitalized patients with acute diarrhoea in Calcutta, India. J Med Microbiol 39: 310-317.
Raychoudhuri, A., Mukherjee, P., Ramamurthy, T., Nandy, R.K., Takeda, Y., Nair, G.B., and Mukhopadhaya, A.K. (2010) Genetic analysis of CTX prophages with special reference to ctxB and rstR alleles of Vibrio cholerae O139 strains isolated from Kolkata over a decade. FEMS Microbiol Lett 303: 107-115.
Waldor, M.K., Tschäpe, H.E., and Mekalanos, J.J. (1996) A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J Bacteriol 178: 4157-4165.
Wayne, P.A. (2010) Clinical and Laboratory Standard Institute. Performance Standards for Antimicrobial Susceptibility Testing; 20th Informational Supplement. CLSI document M100-S20.
Yamai, S., Okitsu, T., Shimada, T., and Katsube, Y. (1997) Distribution of serogroups of Vibrio cholerae non-O1 non-O139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups. Kansenshogaku zasshi 71: 1037.
Yamasaki, S., Nair, G.B., Bhattacharya, S.K., Yamamoto, S., Kurazono, H., and Takeda, Y. (1997) Cryptic appearance of a new clone of Vibrio cholerae serogroup O1 biotype El Tor in Calcutta, India. Microbiol Immunol 41: 1-6.