Measuring Hypocotyl Length in Arabidopsis.
Circadian clock
Circadian mutants
Growth
Hormone signaling
Hypocotyl
Light signaling
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
21
10
2021
pubmed:
22
10
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Circadian clocks allow organisms to synchronize growth to occur at the most optimal time of the day. In plants, the circadian clock controls the timing of hypocotyl (seedling stem) elongation. The activity of the circadian clock subsequently results in hypocotyl elongation being restricted to a small window around dawn and the early morning. Measuring hypocotyl elongation has provided circadian biologists a quick and non-intensive experimental tool to understand the effect of a circadian mutation on plant growth. Furthermore, hypocotyl elongation is also independently regulated by light, temperature, and hormone signaling pathways. Thus, hypocotyl assays can be expanded to investigate the crosstalk between the circadian clock and other extrinsic and intrinsic signaling pathways in controlling plant development. In this chapter we describe the resources and methods required to set up and analyze hypocotyl elongation in Arabidopsis.
Identifiants
pubmed: 34674171
doi: 10.1007/978-1-0716-1912-4_9
doi:
Substances chimiques
Arabidopsis Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
99-106Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Ronald J, Davis S (2017) Making the clock tick: the transcriptional landscape of the plant circadian clock. F1000Res 6:951. (version 1; peer review: 2 approved)
doi: 10.12688/f1000research.11319.1
Choi H, Oh E (2016) PIF4 integrates multiple environmental and hormonal signals for plant growth regulation in Arabidopsis. Mol Cell 39:587
doi: 10.14348/molcells.2016.0126
Nomoto Y, Kubozono S, Yamashino T, Nakamichi N, Mizuno T (2012) Circadian clock- and PIF4-controlled plant growth: a coincidence mechanism directly integrates a hormone signaling network into the photoperiodic control of plant architectures in Arabidopsis thaliana. Plant Cell Physiol 53:1950
doi: 10.1093/pcp/pcs137
Nagel Dawn H, Kay Steve A (2012) Complexity in the wiring and regulation of plant circadian networks. Curr Biol 22:R648
doi: 10.1016/j.cub.2012.07.025
Oakenfull RJ, Davis SJ (2017) Shining a light on the Arabidopsis circadian clock. Plant Cell Environ 40:2571
doi: 10.1111/pce.13033
Anwer MU, Davis A, Davis SJ, Quint M (2020) Photoperiod sensing of the circadian clock is controlled by EARLY FLOWERING 3 and GIGANTEA. Plant J 101:1397
doi: 10.1111/tpj.14604
Hanano S, Domagalska MA, Nagy F, Davis SJ (2006) Multiple phytohormones influence distinct parameters of the plant circadian clock. Genes Cells 11:1381
doi: 10.1111/j.1365-2443.2006.01026.x
Gendreau E, Traas J, Desnos T, Grandjean O, Caboche M, Hofte H (1997) Cellular basis of hypocotyl growth in Arabidopsis thaliana. Plant Physiol 114:295
doi: 10.1104/pp.114.1.295