Smartphone-Based Chemiluminescent Origami µPAD for the Rapid Assessment of Glucose Blood Levels.
biosensor
blood analysis
chemiluminescence
enzyme assay
glucose
hydrogen peroxide
origami
paper-based analytical devices
point of care
smartphone
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
09 Oct 2021
09 Oct 2021
Historique:
received:
09
09
2021
revised:
02
10
2021
accepted:
04
10
2021
entrez:
22
10
2021
pubmed:
23
10
2021
medline:
6
1
2022
Statut:
epublish
Résumé
Microfluidic paper analytical devices (µPADs) represent one of the most appealing trends in the development of simple and inexpensive analytical systems for diagnostic applications at the point of care (POC). Herein, we describe a smartphone-based origami µPAD for the quantitative determination of glucose in blood samples based on the glucose oxidase-catalyzed oxidation of glucose leading to hydrogen peroxide, which is then detected by means of the luminol/hexacyanoferrate(III) chemiluminescent (CL) system. By exploiting the foldable µPAD format, a two-step analytical procedure has been implemented. First, the diluted blood sample was added, and hydrogen peroxide was accumulated, then the biosensor was folded, and a transport buffer was added to bring hydrogen peroxide in contact with CL reagents, thus promoting the CL reaction. To enable POC applicability, the reagents required for the assay were preloaded in the µPAD so that no chemicals handling was required, and a 3D-printed portable device was developed for measuring the CL emission using the smartphone's CMOS camera. The µPAD was stable for 30-day storage at room temperature and the assay, displaying a limit of detection of 10 µmol L
Identifiants
pubmed: 34677337
pii: bios11100381
doi: 10.3390/bios11100381
pmc: PMC8533763
pii:
doi:
Substances chimiques
Blood Glucose
0
Hydrogen Peroxide
BBX060AN9V
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Italian Ministry of University and Research
ID : PRIN2017 project Prot. 2017Y2PAB8
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