Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China.
Maedi/Visna virus
next-generation sequencing
phylogenetic analysis
sheep lung
Journal
Journal of veterinary science
ISSN: 1976-555X
Titre abrégé: J Vet Sci
Pays: Korea (South)
ID NLM: 100964185
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
14
04
2021
revised:
18
05
2021
accepted:
08
07
2021
pubmed:
27
10
2021
medline:
4
2
2022
entrez:
26
10
2021
Statut:
ppublish
Résumé
Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
Sections du résumé
BACKGROUND
BACKGROUND
Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide.
OBJECTIVES
OBJECTIVE
In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated.
METHODS
METHODS
Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis.
RESULTS
RESULTS
A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%-86.8% and 67.7%-75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5).
CONCLUSIONS
CONCLUSIONS
The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
Identifiants
pubmed: 34697919
pii: 22.e66
doi: 10.4142/jvs.2021.22.e66
pmc: PMC8636652
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e66Subventions
Organisme : Inner Mongolia Grassland Innovative Talent Team Project
ID : 20151031
Pays : China
Organisme : Inner Mongolia Technology Application Research Project
ID : 2019GG240
Pays : China
Organisme : National Natural Science Foundation of China
ID : 31760721
Pays : China
Organisme : National Natural Science Foundation of China
ID : 32072819
Pays : China
Informations de copyright
© 2021 The Korean Society of Veterinary Science.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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