Live imaging of retinotectal mapping reveals topographic map dynamics and a previously undescribed role for Contactin 2 in map sharpening.
Adhesion molecule
Axon guidance
Refinement
Visual system
Zebrafish
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
15 11 2021
15 11 2021
Historique:
received:
03
03
2021
accepted:
07
10
2021
pubmed:
27
10
2021
medline:
3
2
2022
entrez:
26
10
2021
Statut:
ppublish
Résumé
Organization of neuronal connections into topographic maps is essential for processing information. Yet, our understanding of topographic mapping has remained limited by our inability to observe maps forming and refining directly in vivo. Here, we used Cre-mediated recombination of a new colorswitch reporter in zebrafish to generate the first transgenic model allowing the dynamic analysis of retinotectal mapping in vivo. We found that the antero-posterior retinotopic map forms early but remains dynamic, with nasal and temporal retinal axons expanding their projection domains over time. Nasal projections initially arborize in the anterior tectum but progressively refine their projection domain to the posterior tectum, leading to the sharpening of the retinotopic map along the antero-posterior axis. Finally, using a CRISPR-mediated mutagenesis approach, we demonstrate that the refinement of nasal retinal projections requires the adhesion molecule Contactin 2. Altogether, our study provides the first analysis of a topographic map maturing in real time in a live animal and opens new strategies for dissecting the molecular mechanisms underlying precise topographic mapping in vertebrates.
Identifiants
pubmed: 34698769
pii: 272618
doi: 10.1242/dev.199584
pmc: PMC8645211
pii:
doi:
Substances chimiques
Contactin 2
0
Zebrafish Proteins
0
cntn2 protein, zebrafish
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS109197
Pays : United States
Informations de copyright
© 2021. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
Références
Front Neural Circuits. 2017 Jan 10;10:111
pubmed: 28119574
Cell. 2020 Sep 17;182(6):1623-1640.e34
pubmed: 32946783
J Cell Biol. 1991 Nov;115(4):1113-26
pubmed: 1720120
PLoS One. 2019 Jun 20;14(6):e0218667
pubmed: 31220164
Int J Mol Sci. 2020 Jul 21;21(14):
pubmed: 32708320
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11403-8
pubmed: 11027340
Development. 2011 Jan;138(1):169-77
pubmed: 21138979
Science. 2014 May 23;344(6186):904-9
pubmed: 24855269
Dev Cell. 2019 Dec 2;51(5):645-657.e4
pubmed: 31708433
Development. 2005 Jul;132(13):2955-67
pubmed: 15930106
J Neurosci. 2006 Mar 29;26(13):3594-603
pubmed: 16571768
Proc Natl Acad Sci U S A. 1963 Oct;50:703-10
pubmed: 14077501
J Neurosci. 1995 Apr;15(4):2935-47
pubmed: 7722638
Methods. 2017 May 15;121-122:16-28
pubmed: 28351759
Development. 1996 Dec;123:439-50
pubmed: 9007261
J Neurosci. 1990 Nov;10(11):3615-26
pubmed: 2230950
Neural Dev. 2008 Mar 17;3:7
pubmed: 18346270
Neuron. 1995 Jun;14(6):1165-79
pubmed: 7541632
Nat Neurosci. 2011 Nov 18;15(2):298-307
pubmed: 22179110
Development. 1996 Dec;123:415-25
pubmed: 9007259
J Neurosci. 1998 Nov 15;18(22):9181-91
pubmed: 9801358
Dev Dyn. 2009 Dec;238(12):3168-74
pubmed: 19890916
Mol Cell Neurosci. 2001 Feb;17(2):398-414
pubmed: 11178876
Neuron. 2013 Apr 10;78(1):49-56
pubmed: 23583107
Dev Biol. 2001 Dec 1;240(1):123-42
pubmed: 11784051
PLoS Biol. 2005 Jan;3(1):e7
pubmed: 15630479
J Cell Biol. 1995 Nov;131(4):1067-81
pubmed: 7490283
Genome Res. 2012 Oct;22(10):2043-53
pubmed: 22593555
J Neurosci. 2005 Jul 20;25(29):6929-38
pubmed: 16033903
Mol Biol Cell. 2000 May;11(5):1523-33
pubmed: 10793132
Front Neural Circuits. 2016 Sep 05;10:71
pubmed: 27656131
Neuron. 2005 Dec 8;48(5):797-809
pubmed: 16337917
Nature. 2005 Apr 21;434(7036):1022-6
pubmed: 15846347
Development. 2000 Dec;127(23):5033-45
pubmed: 11060230
J Neurosci. 2006 Dec 13;26(50):12873-84
pubmed: 17167078
Elife. 2020 Oct 12;9:
pubmed: 33044168
Nat Cell Biol. 2008 Mar;10(3):283-94
pubmed: 18278038
Am J Hum Genet. 2008 May;82(5):1178-84
pubmed: 18423520
Nat Genet. 2012 Jan 08;44(2):148-56
pubmed: 22231485
Dev Dyn. 2007 Nov;236(11):3088-99
pubmed: 17937395
Development. 2008 Aug;135(14):2361-71
pubmed: 18550718
J Cell Biol. 2000 May 15;149(4):951-68
pubmed: 10811834
J Neurosci. 2001 May 15;21(10):3542-8
pubmed: 11331383
J Neurosci. 2013 Nov 13;33(46):18208-18
pubmed: 24227729
Dev Neurobiol. 2015 Jun;75(6):542-56
pubmed: 25492632
Adv Neurobiol. 2014;8:165-94
pubmed: 25300137
J Biol Chem. 2001 Dec 7;276(49):46553-61
pubmed: 11581273
Dev Genes Evol. 2009 Oct;219(9-10):481-96
pubmed: 20112453
Med Image Anal. 2001 Jun;5(2):111-26
pubmed: 11516706
Invest Ophthalmol Vis Sci. 2015 Jan 08;56(2):883-91
pubmed: 25574057
Curr Opin Neurobiol. 2013 Feb;23(1):119-26
pubmed: 23246238
PLoS Genet. 2012;8(8):e1002861
pubmed: 22916025
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Exp Eye Res. 2019 Jul;184:234-242
pubmed: 31075224
Cell Rep. 2020 Jan 28;30(4):1164-1177.e7
pubmed: 31995756
Dev Neurobiol. 2015 Jun;75(6):621-40
pubmed: 25787992
Brain Res Dev Brain Res. 1990 Dec 1;57(1):29-35
pubmed: 2090369
J Neurogenet. 2017 Sep;31(3):61-69
pubmed: 28797199
Dev Genes Evol. 2001 Jul;211(7):338-49
pubmed: 11466530
J Comp Neurol. 1999 Feb 22;404(4):515-36
pubmed: 9987995
Cell Tissue Res. 2001 Nov;306(2):199-207
pubmed: 11702231
J Cell Biol. 1996 Dec;135(6 Pt 1):1593-607
pubmed: 8978825
J Neurosci. 1999 Nov 15;19(22):9996-10003
pubmed: 10559407
Genome Res. 2012 Dec;22(12):2356-67
pubmed: 22722344
J Neurosci. 2012 Jul 25;32(30):10370-82
pubmed: 22836270
Dev Dyn. 1995 Jul;203(3):253-310
pubmed: 8589427
J Neurosci. 1985 May;5(5):1132-43
pubmed: 3873522
Neural Dev. 2008 Feb 20;3:6
pubmed: 18289389
J Neurosci. 2004 Aug 18;24(33):7387-99
pubmed: 15317864
Genome Biol. 2007;8 Suppl 1:S7
pubmed: 18047699
IEEE Pac Vis Symp. 2012;:201-208
pubmed: 23584131
Development. 2005 Nov;132(22):4951-62
pubmed: 16236770
J Neurobiol. 1994 Jul;25(7):781-96
pubmed: 8089656
Dev Dyn. 2008 Sep;237(9):2460-5
pubmed: 18729206
Nat Neurosci. 2001 Nov;4(11):1093-101
pubmed: 11593233
Nature. 2011 Feb 10;470(7333):279-83
pubmed: 21160473
Mech Dev. 1999 Feb;80(2):197-201
pubmed: 10072788
Eur J Neurosci. 2005 Jul;22(1):39-49
pubmed: 16029194
Ophthalmic Genet. 2011 Jun;32(2):114-7
pubmed: 21417677
J Cell Biol. 2020 Jun 1;219(6):
pubmed: 32346721
J Biol Chem. 2004 Dec 10;279(50):52744-52
pubmed: 15466474
J Neurosci. 2011 Mar 2;31(9):3384-99
pubmed: 21368050
Neuron. 2014 Nov 19;84(4):740-52
pubmed: 25451192
Science. 2003 Jul 4;301(5629):66-70
pubmed: 12843386
Nature. 2008 Apr 17;452(7189):892-5
pubmed: 18368050
J Neurosci. 2010 Aug 11;30(32):10939-51
pubmed: 20702722
Nat Protoc. 2008;3(1):59-69
pubmed: 18193022
Dev Biol. 1999 Mar 15;207(2):309-21
pubmed: 10068465
Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):E5105-13
pubmed: 25385606
J Neurobiol. 2000 Feb 15;42(3):303-14
pubmed: 10645970
J Neurosci. 2004 Apr 21;24(16):4030-42
pubmed: 15102918
Genome Res. 2002 Jun;12(6):996-1006
pubmed: 12045153
Genomics. 1998 May 15;50(1):61-8
pubmed: 9628823
J Biol Chem. 2004 Apr 16;279(16):16044-9
pubmed: 14761957
Cell Adh Migr. 2017 Sep 3;11(5-6):524-531
pubmed: 28135886
J Neurosci. 1992 Aug;12(8):3257-71
pubmed: 1494955
Nat Genet. 2007 Mar;39(3):311-8
pubmed: 17277777
Mech Dev. 2018 Aug;152:1-12
pubmed: 29777776
Cell Rep. 2020 Oct 6;33(1):108220
pubmed: 33027659
J Neurosci. 2008 Oct 22;28(43):11015-23
pubmed: 18945909
Neuron. 1990 Aug;5(2):159-71
pubmed: 2383399
Neuron. 2007 Jan 4;53(1):65-77
pubmed: 17196531
Curr Opin Neurobiol. 2017 Feb;42:136-143
pubmed: 28088066
Eur J Neurosci. 2001 Aug;14(4):645-56
pubmed: 11556889
BMC Dev Biol. 2009 Apr 20;9:27
pubmed: 19379485
J Neurosci. 1994 Feb;14(2):486-98
pubmed: 7905512
Exp Eye Res. 2012 Dec;105:34-42
pubmed: 23068565
Neuron. 2007 Jul 19;55(2):231-45
pubmed: 17640525
Neuron. 2014 Dec 3;84(5):1049-64
pubmed: 25466916
J Neurophysiol. 2015 Aug;114(2):1146-57
pubmed: 26133798
Development. 2008 Sep;135(17):2865-71
pubmed: 18653554
J Neurosci. 2001 Oct 1;21(19):7517-25
pubmed: 11567041
EMBO J. 2019 Sep 16;38(18):e100811
pubmed: 31436334
Annu Rev Neurosci. 2013 Jul 8;36:51-77
pubmed: 23642132
J Cell Biol. 1998 Dec 14;143(6):1673-90
pubmed: 9852159
Mech Dev. 2000 Dec;99(1-2):123-37
pubmed: 11091080
Development. 2012 Jan;139(2):359-72
pubmed: 22186726
J Neurosci. 1988 Dec;8(12):4513-30
pubmed: 2848935
Proc Natl Acad Sci U S A. 2020 May 19;117(20):10636-10638
pubmed: 32366647
J Neurobiol. 1996 Jan;29(1):65-74
pubmed: 8748372
J Neurosci. 2016 Mar 30;36(13):3871-86
pubmed: 27030771
Development. 2003 Nov;130(21):5203-15
pubmed: 12954716
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
J Neurosci. 2006 Mar 29;26(13):3604-14
pubmed: 16571769
Neuron. 2002 Jan 31;33(3):357-67
pubmed: 11832224
Dev Biol. 1994 Jan;161(1):251-62
pubmed: 7904968
Adv Genet. 2016;95:65-87
pubmed: 27503354
J Comp Neurol. 2013 Apr 15;521(6):1409-29
pubmed: 23124714
Methods Cell Biol. 2010;100:3-26
pubmed: 21111212
Dev Biol. 2009 Jul 15;331(2):300-10
pubmed: 19450575
BMC Bioinformatics. 2017 May 26;18(1):280
pubmed: 28549411
Neuron. 1996 Feb;16(2):255-60
pubmed: 8789941
Development. 1996 Dec;123:399-413
pubmed: 9007258
Curr Opin Neurobiol. 2014 Feb;24(1):7-12
pubmed: 24492072
Semin Cell Dev Biol. 2014 Nov;35:126-35
pubmed: 25084320