Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis.
antibody
biomarker
immunoassay
multiplex
next-generation sequencing
plasma
proteomics
proximity extension assay
serum
Journal
Molecular & cellular proteomics : MCP
ISSN: 1535-9484
Titre abrégé: Mol Cell Proteomics
Pays: United States
ID NLM: 101125647
Informations de publication
Date de publication:
2021
2021
Historique:
received:
20
05
2021
revised:
14
10
2021
accepted:
21
10
2021
pubmed:
30
10
2021
medline:
25
3
2022
entrez:
29
10
2021
Statut:
ppublish
Résumé
Understanding the dynamics of the human proteome is crucial for developing biomarkers to be used as measurable indicators for disease severity and progression, patient stratification, and drug development. The Proximity Extension Assay (PEA) is a technology that translates protein information into actionable knowledge by linking protein-specific antibodies to DNA-encoded tags. In this report we demonstrate how we have combined the unique PEA technology with an innovative and automated sample preparation and high-throughput sequencing readout enabling parallel measurement of nearly 1500 proteins in 96 samples generating close to 150,000 data points per run. This advancement will have a major impact on the discovery of new biomarkers for disease prediction and prognosis and contribute to the development of the rapidly evolving fields of wellness monitoring and precision medicine.
Identifiants
pubmed: 34715355
pii: S1535-9476(21)00140-7
doi: 10.1016/j.mcpro.2021.100168
pmc: PMC8633680
pii:
doi:
Substances chimiques
Biomarkers
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100168Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest All authors are employees of Olink Proteomics AB commercializing the described method. An author may or may not be named as an inventor on current patents owned and controlled by Olink Proteomics AB, https://www.olink.com/patents/, as amended from time to time. All authors have financial interest in Olink Holding AB.
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