NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration.
Animal Fins
/ metabolism
Animals
Cell Proliferation
Forkhead Transcription Factors
/ metabolism
Gene Expression Regulation, Developmental
Genes, erbB
/ genetics
Larva
Macrophages
/ metabolism
Neural Crest
/ metabolism
Neuregulin-1
/ genetics
Regeneration
/ genetics
Signal Transduction
/ genetics
Stem Cells
Zebrafish
/ genetics
Zebrafish Proteins
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 11 2021
03 11 2021
Historique:
received:
07
10
2020
accepted:
07
09
2021
entrez:
4
11
2021
pubmed:
5
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.
Identifiants
pubmed: 34732706
doi: 10.1038/s41467-021-26422-5
pii: 10.1038/s41467-021-26422-5
pmc: PMC8566576
doi:
Substances chimiques
Forkhead Transcription Factors
0
Neuregulin-1
0
Zebrafish Proteins
0
foxd3 protein, zebrafish
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6336Informations de copyright
© 2021. The Author(s).
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