Determining translocation orientations of nucleic acid helicases.
DNA replication
MCM helicase
Orientation
Polarity
Translocation
Unwinding
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
06
09
2021
revised:
29
10
2021
accepted:
02
11
2021
pubmed:
11
11
2021
medline:
22
6
2022
entrez:
10
11
2021
Statut:
ppublish
Résumé
Helicase enzymes translocate along an RNA or DNA template with a defined polarity to unwind, separate, or remodel duplex strands for a variety of genome maintenance processes. Helicase mutations are commonly associated with a variety of diseases including aging, cancer, and neurodegeneration. Biochemical characterization of these enzymes has provided a wealth of information on the kinetics of unwinding and substrate preferences, and several high-resolution structures of helicases alone and bound to oligonucleotides have been solved. Together, they provide mechanistic insights into the structural translocation and unwinding orientations of helicases. However, these insights rely on structural inferences derived from static snapshots. Instead, continued efforts should be made to combine structure and kinetics to better define active translocation orientations of helicases. This review explores many of the biochemical and biophysical methods utilized to map helicase binding orientation to DNA or RNA substrates and includes several time-dependent methods to unequivocally map the active translocation orientation of these enzymes to better define the active leading and trailing faces.
Identifiants
pubmed: 34758393
pii: S1046-2023(21)00250-4
doi: 10.1016/j.ymeth.2021.11.001
pmc: PMC9076756
mid: NIHMS1756217
pii:
doi:
Substances chimiques
Nucleic Acids
0
RNA
63231-63-0
DNA
9007-49-2
DNA Helicases
EC 3.6.4.-
Types de publication
Journal Article
Review
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
160-171Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM135791
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
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