Reproducible and efficient new method of RNA 3'-end labelling by CutA nucleotidyltransferase-mediated CC-tailing.
3ʹ-end RNA labelling
CutA
Dis3
T4 RNA ligase I
Xrn1
dsRNA
exoribonuclease directionality
nucleotidyltransferase
radioactive nucleotides
ssRNA
Journal
RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328
Informations de publication
Date de publication:
12 11 2021
12 11 2021
Historique:
pubmed:
13
11
2021
medline:
11
3
2022
entrez:
12
11
2021
Statut:
ppublish
Résumé
Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated into the body or at the 5' or 3' terminus of the molecule can serve as probes in hybridization-based analyses of
Identifiants
pubmed: 34766865
doi: 10.1080/15476286.2021.1999104
pmc: PMC8791449
doi:
Substances chimiques
Nucleotides
0
Phosphorus Radioisotopes
0
RNA
63231-63-0
Cytidine Triphosphate
65-47-4
Phosphorus-32
690284A407
Nucleotidyltransferases
EC 2.7.7.-
RNA Ligase (ATP)
EC 6.5.1.3
Uridine Triphosphate
UT0S826Z60
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
623-639Références
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