Molecular identification and functional verification of SPL9 and SPL15 of Lilium.
Arabidopsis
/ genetics
Flowers
/ genetics
Gene Expression Regulation, Developmental
/ genetics
Gene Expression Regulation, Plant
Genes, Plant
/ physiology
Lilium
/ classification
Phenotype
Plant Development
Plant Proteins
/ genetics
Plant Roots
/ genetics
Plants, Genetically Modified
Nicotiana
/ genetics
Transcription Factors
/ genetics
Lilium
Molecular mechanisms
Phase transition
SPL15
SPL9
Journal
Molecular genetics and genomics : MGG
ISSN: 1617-4623
Titre abrégé: Mol Genet Genomics
Pays: Germany
ID NLM: 101093320
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
09
03
2021
accepted:
30
10
2021
pubmed:
16
11
2021
medline:
8
2
2022
entrez:
15
11
2021
Statut:
ppublish
Résumé
The transformation of plants from juveniles to adults is a key process in plant growth and development, and the main regulatory factors are miR156 and SQUAMOSA promoter binding protein-like (SPL) transcription factors. Lilium is an ornamental bulb, but it has a long maturation time. In this experiment, Lilium bulbs were subjected to a temperature treatment of 15 °C for 4 weeks to initiate vegetative phase change. Transmission electron microscopy indicated the cell wall of bud core tissue undergoing vegetative phase change became thinner, the starch grains were reduced, and the growth of the juvenile stage was accelerated. The key transcription factors LbrSPL9 and LbrSPL15 were cloned, and the phylogenetic analysis showed they possessed high homology with other plant SPLs. Subcellular localization and transcription activation experiments confirmed LbrSPL9 and LbrSPL15 were mainly located in the nucleus and exhibited transcriptional activity. The results of in situ hybridization showed the expression levels of LbrSPL9 and LbrSPL15 were increased after temperature change treatment. The functional verification experiment of the transgenic plants confirmed that the overexpression of LbrSPL9 and LbrSPL15 could shorten maturation time. These findings help elucidate the regulatory mechanisms of phase transition in Lilium and provide a reference for breeding research in other bulbous flowers.
Identifiants
pubmed: 34779936
doi: 10.1007/s00438-021-01832-8
pii: 10.1007/s00438-021-01832-8
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
63-74Subventions
Organisme : Beijing Natural Science Fund-Municipal Education Commission
ID : KZ201810020029
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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