Genetic engineering of the acidophilic chemolithoautotroph Acidithiobacillus ferrooxidans.
Acidithiobacillus ferrooxidans
iron oxidation
sulfur oxidation
synthetic biology
Journal
Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
15
07
2021
revised:
05
10
2021
accepted:
06
10
2021
pubmed:
20
11
2021
medline:
18
5
2022
entrez:
19
11
2021
Statut:
ppublish
Résumé
There are several natural and anthropomorphic environments where iron- and/or sulfur-oxidizing bacteria thrive in extremely acidic conditions. These acidophilic chemolithautotrophs play important roles in biogeochemical iron and sulfur cycles, are critical catalysts for industrial metal bioleaching operations, and have underexplored potential in future biotechnological applications. However, their unique growth conditions complicate the development of genetic techniques. Over the past few decades genetic tools have been successfully developed for Acidithiobacillus ferrooxidans, which serves as a model organism that exhibits both iron- and sulfur-oxidizing capabilities. Conjugal transfer of plasmids has enabled gene overexpression, gene knockouts, and some preliminary metabolic engineering. We highlight the development of genetic systems and recent genetic engineering of A. ferrooxidans, and discuss future perspectives.
Identifiants
pubmed: 34794837
pii: S0167-7799(21)00235-3
doi: 10.1016/j.tibtech.2021.10.004
pii:
doi:
Substances chimiques
Sulfur
70FD1KFU70
Iron
E1UOL152H7
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
677-692Informations de copyright
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