Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species.
Chromophore
Flavin mononucleotide
Flavin-based fluorescent protein
LOV domain
Protein–ligand interactions
Riboflavin
Thermal stability
Journal
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
ISSN: 1474-9092
Titre abrégé: Photochem Photobiol Sci
Pays: England
ID NLM: 101124451
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
06
09
2021
accepted:
08
11
2021
pubmed:
20
11
2021
medline:
14
1
2022
entrez:
19
11
2021
Statut:
ppublish
Résumé
Light-oxygen-voltage (LOV) domains are common photosensory modules that found many applications in fluorescence microscopy and optogenetics. Here, we show that the Chloroflexus aggregans LOV domain can bind different flavin species (lumichrome, LC; riboflavin, RF; flavin mononucleotide, FMN; flavin adenine dinucleotide, FAD) during heterologous expression and that its physicochemical properties depend strongly on the nature of the bound flavin. We show that whereas the dissociation constants for different chromophores are similar, the melting temperature of the protein reconstituted with single flavin species varies from ~ 60 °C for LC to ~ 81 °C for FMN, and photobleaching half-times vary almost 100-fold. These observations serve as a caution for future studies of LOV domains in non-native conditions yet raise the possibility of fine-tuning various properties of LOV-based fluorescent probes and optogenetic tools by manipulating the chromophore composition.
Identifiants
pubmed: 34796467
doi: 10.1007/s43630-021-00138-3
pii: 10.1007/s43630-021-00138-3
doi:
Substances chimiques
Flavin-Adenine Dinucleotide
146-14-5
Flavin Mononucleotide
7N464URE7E
Oxygen
S88TT14065
Riboflavin
TLM2976OFR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1645-1656Subventions
Organisme : Russian Science Foundation
ID : 21-64-00018
Informations de copyright
© 2021. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
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