Development of a microchip capillary electrophoresis method for determination of the purity and integrity of mRNA in lipid nanoparticle vaccines.
Covid-19
Lipid nanoparticles
Microchip CE
mRNA vaccine
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
revised:
01
11
2021
received:
30
08
2021
accepted:
06
11
2021
pubmed:
23
11
2021
medline:
18
5
2022
entrez:
22
11
2021
Statut:
ppublish
Résumé
Messenger RNA (mRNA)-based vaccines are advantageous because they can be relatively quicker and more cost efficient to manufacture compared to other traditional vaccine products. Lipid nanoparticles have three common purposes: delivery, self-adjuvanting properties, and mRNA protection. Faster vaccine development requires an efficient and fast assay to monitor mRNA purity and integrity. Microchip CE is known to be a robust technology that is capable of rapid separation. Here, we describe the development and optimization of a purity and integrity assay for mRNA-based vaccines encapsulated in lipid nanoparticles using commercial microchip-based separation. The analytical parameters of the optimized assay were assessed and the method is a stability indicating assay.
Identifiants
pubmed: 34806186
doi: 10.1002/elps.202100272
pmc: PMC9011815
doi:
Substances chimiques
Lipid Nanoparticles
0
Liposomes
0
RNA, Messenger
0
Vaccines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1101-1106Informations de copyright
© 2021 Merck Sharp & Dohme Corp. Electrophoresis published by Wiley-VCH GmbH.
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