Richard R Rustandi

Affiliations

Vaccine Research Analytical Development, Merck & Co., Inc., Kenilworth, NJ, USA. Electronic address: richard_rustandi@merck.com.

Publications (37)

Development of an ADP-ribosylation assay for residual toxicity in C. difficile binary toxin CDTa using automated capillary western blot.

Journal of pharmaceutical and biomedical analysis

Avec: Richard R Rustandi , Melissa Hamm

Determination of lipid content and stability in lipid nanoparticles using ultra high-performance liquid chromatography in combination with a Corona Charged Aerosol Detector.

Electrophoresis

Avec: Caleb Kinsey , Tian Lu , Alyssa Deiss , Kim Vuolo , Lee Klein , Richard R Rustandi , John W Loughney

Reverse-Phase Ultra-Performance Chromatography Method for Oncolytic Coxsackievirus Viral Protein Separation and Empty to Full Capsid Quantification.

Human gene therapy

Avec: James Z Deng , Richard R Rustandi , Damon Barbacci , Andrew R Swartz , Amanda Gulasarian , John W Loughney

Functional profiling of Covid 19 vaccine candidate by flow virometry.

Vaccine

Avec: Ashley Prout , Richard R Rustandi , Christopher Tubbs , Michael A Winters , Philip McKenna , Josef Vlasak

Regeneration of Capto Core 700 resin through high throughput and laboratory scale studies and impact on production of a SARS-CoV-2 vaccine candidate.

Biotechnology journal

Avec: Spyridon Konstantinidis , Seth R Reinhart , Christine Castagna , Murphy R Poplyk , Richard R Rustandi , Kristen L Flor , Jillian Acevedo-Skrip , Rachel Thompson , Christopher J Wang , Sheng-Ching Wang , Michael A Winters

Adaptation of an rVSV Ebola vaccine purification process for rapid development of a viral vaccine candidate for SARS-CoV-2.

Biotechnology journal

Avec: Laura E Kuczynski , James R Shallow , Matthew P Watson , Michael L Homsy , Thomas Svab , Ashley Gruber , Richard R Rustandi , Jianfang Hu , Michael A Winters

Identification of adipocyte plasma membrane-associated protein as a novel modulator of human cytomegalovirus infection.

PLoS pathogens

Avec: Xiaohua Ye , Xun Gui , Daniel C Freed , Zhiqiang Ku , Leike Li , Yuanzhi Chen , Wei Xiong , Xuejun Fan , Hang Su , Xi He , Richard R Rustandi , John W Loughney , Ningning Ma , Amy S Espeseth , Jian Liu , Hua Zhu , Dai Wang , Ningyan Zhang , Tong-Ming Fu , Zhiqiang An

High-Resolution capillary electrophoresis separation of large RNA under non-aqueous conditions.

Journal of chromatography. A

Avec: Tian Lu , Lee J Klein , Sha Ha , Richard R Rustandi

Recent advances in isoelectric focusing of proteins and peptides.

Journal of chromatography. A

Avec: Lily Farmerie , Richard R Rustandi , John W Loughney , Mohamed Dawod

Development of a microchip capillary electrophoresis method for determination of the purity and integrity of mRNA in lipid nanoparticle vaccines.

Electrophoresis

Avec: Jessica Raffaele , John W Loughney , Richard R Rustandi

Development and qualification of an automated capillary Western method for the identification of polysaccharide serotypes in pneumococcal conjugate vaccine (PCV).

Journal of pharmaceutical and biomedical analysis

Avec: Alyssa Deiss , John W Loughney , Richard R Rustandi , Kimberly Vuolo , Megan A Mackey

Determining the Oxidation Mechanism through Radical Intermediates in Polysorbates 80 and 20 by Electron Paramagnetic Resonance Spectroscopy.

Pharmaceuticals (Basel, Switzerland)

Avec: Adam T Sutton , Richard R Rustandi

Flow virometry for process monitoring of live virus vaccines-lessons learned from ERVEBO.

Scientific reports

Avec: Geoffri Ricci , Kevin Minsker , Austin Kapish , James Osborn , Sha Ha , Joseph Davide , Joseph P Califano , Darrell Sehlin , Richard R Rustandi , Lawrence W Dick , Josef Vlasak , Timothy D Culp , Andreas Baudy , Edward Bell , Malini Mukherjee

Characterization of rVSVΔG-ZEBOV-GP glycoproteins using automated capillary western blotting.

Vaccine

Avec: Kevin Minsker , Richard R Rustandi , Sha Ha , John W Loughney

Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus-Ligand Binding.

Viruses

Avec: Shruthi Murali , Richard R Rustandi , Xiwei Zheng , Anne Payne , Liang Shang

Membrane binding and pore formation is Ca

bioRxiv : the preprint server for biology

Avec: Dinendra L Abeyawardhane , Spiridon E Sevdalis , Kaylin A Adipietro , Raquel Godoy-Ruiz , Kristen M Varney , Izza F Nawaz , Alejandro X Spittel , Richard R Rustandi , Vitalii I Silin , Amedee des Georges , Edwin Pozharski , David J Weber

Monoclonal Antibody Reagent Stability and Expiry Recommendation Combining Experimental Data with Mathematical Modeling.

The AAPS journal

Avec: Malorie Fink , Erin Marie Cannon , Carl Hofmann , Nisarg Patel , Cindy Pauley , Matthew Troutman , Richard R Rustandi , Mary Shank-Retzlaff , John Loughney , Thorsten Verch

Structure of the cell-binding component of the

Proceedings of the National Academy of Sciences of the United States of America

Avec: Xingjian Xu , Raquel Godoy-Ruiz , Kaylin A Adipietro , Christopher Peralta , Danya Ben-Hail , Kristen M Varney , Mary E Cook , Braden M Roth , Paul T Wilder , Thomas Cleveland , Alexander Grishaev , Heather M Neu , Sarah L J Michel , Wenbo Yu , Dorothy Beckett , Richard R Rustandi , Catherine Lancaster , John W Loughney , Adam Kristopeit , Sianny Christanti , Jessica W Olson , Alexander D MacKerell , Amedee des Georges , Edwin Pozharski , David J Weber

Characterization of gH/gL/pUL128-131 pentameric complex, gH/gL/gO trimeric complex, gB and gM/gN glycoproteins in a human cytomegalovirus using automated capillary western blots.

Vaccine

Avec: Richard R Rustandi , John W Loughney , Liang Shang , Shiyi Wang , Cindy J Pauley , Sianny Christanti , Adam Kristopeit , Timothy D Culp

SEC coupled with in-line multiple detectors for the characterization of an oncolytic Coxsackievirus.

Molecular therapy oncolytics

Avec: James Z Deng , Richard R Rustandi , Andrew Swartz , Yvonne Shieh , Jack B Baker , Josef Vlasak , Shiyi Wang , John W Loughney

Application of cation exchange chromatography in bind and elute and flowthrough mode for the purification of enteroviruses.

Journal of chromatography. A

Avec: Spyridon Konstantinidis , Murphy R Poplyk , Andrew R Swartz , Richard R Rustandi , Rachel Thompson , Sheng-Ching Wang

Analytical technology development to monitor the stability of Polysaccharide-Protein conjugate vaccines.

Vaccine

Avec: William J Smith , Patrick L Ahl , Bei Wang , Melissa Hamm , Richard R Rustandi , Michael A Winters , Jeffrey T Blue

Reactivity characterization of bromoacetyl derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB® by NMR spectroscopy.

Vaccine

Avec: Richard R Rustandi , Qiuwei Xu

Detection of residual T7 RNA polymerase used in mRNA in vitro transcription by Simple Western.

Electrophoresis

Avec: Yvonne Shieh , Andrew R Swartz , Richard R Rustandi

Monitoring bromide loss in bromoacetyl-derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB® by capillary electrophoresis and NMR spectroscopy.

Vaccine

Avec: Richard R Rustandi , Melissa Hamm , Matthew Onimus , Yue Yuan , Carrie L Anderson , Chengli Zong

Extensive Characterization of Polysorbate 80 Oxidative Degradation Under Stainless Steel Conditions.

Journal of pharmaceutical sciences

Avec: Xiwei Zheng , Adam T Sutton , Rong-Sheng Yang , Danielle V Miller , Becca Pagels , Richard R Rustandi , Jonathan Welch , Anne Payne , Mark Haverick

Development of an imaged capillary isoelectric focusing method for characterizing the surface charge of mRNA lipid nanoparticle vaccines.

Electrophoresis

Avec: John W Loughney , Kevin Minsker , Sha Ha , Richard R Rustandi

The Importance of Therapeutically Targeting the Binary Toxin from

International journal of molecular sciences

Avec: Dinendra L Abeyawardhane , Raquel Godoy-Ruiz , Kaylin A Adipietro , Kristen M Varney , Richard R Rustandi , Edwin Pozharski , David J Weber

Polysorbate 80 and histidine quantitative analysis by NMR in the presence of virus-like particles.

Electrophoresis

Avec: Richard R Rustandi

Correlating Stability-Indicating Biochemical and Biophysical Characteristics with In Vitro Cell Potency in mRNA LNP Vaccine.

Vaccines

Avec: Xin Tong , Jessica Raffaele , Katrina Feller , Geethanjali Dornadula , James Devlin , David Boyd , John W Loughney , Jon Shanter , Richard R Rustandi

Development of a capillary zone electrophoresis method to monitor magnesium ion consumption during in vitro transcription for mRNA production.

Analytical and bioanalytical chemistry

Avec: Ying Wang , Li Li , Adam T Sutton , Qiang Tu , Kaixi Zhao , Emily Wen , James Osborn , Andrew Singh , Matthew J Gunsch , Richard Rianto Rustandi , David Foley , Yu He

Binding of Coxsackievirus A21 procapsids to immobilized glutathione depends on cell culture conditions during infection.

Virology

Avec: Andrew R Swartz , Yvonne Shieh , Amanda Gulasarian , Jessica W Olson , Richard R Rustandi

Recent advances in capillary gel electrophoresis for the analysis of proteins.

Journal of chromatography. A

Avec: Rijul Bhimwal , Richard R Rustandi , Anne Payne , Mohamed Dawod

Quantitation of Coxsackievirus A21 Viral Proteins in Mixtures of Empty and Full Capsids Using Capillary Western.

Human gene therapy

Avec: Paul F Gillespie , Richard R Rustandi , Andrew R Swartz , Liang Shang , Jessica Raffaele , Ashley Prout , Nicholas Cunningham , Mohamed Dawod , James Z Deng , Shiyi Wang , Jessica Olson , Yvonne Shieh , John W Loughney

Understanding the Spike Protein in COVID-19 Vaccine in Recombinant Vesicular Stomatitis Virus (rVSV) Using Automated Capillary Western Blots.

ACS omega

Avec: Paul F Gillespie , Yanjie Wang , Carl Hofmann , Laura E Kuczynski , Michael A Winters , Jennifer L Teyral , Christopher M Tubbs , Kelsey Shiflett , Nisarg Patel , Richard R Rustandi

Quantitation of host cell proteins in biopharmaceuticals from chinese hamster ovarian and vero cell lines using capillary electrophoresis western blots.

Journal of pharmaceutical and biomedical analysis

Avec: Cara Pearson , Yi Wang , Eman Alkurdi , Young Zhang , Kuo Yin , Richard R Rustandi , John W Loughney

Development and qualification of cell-based relative potency assay for a human respiratory syncytial virus (RSV) mRNA vaccine.

Journal of pharmaceutical and biomedical analysis

Avec: Hualin Helen Li , Jenny Xu , Li He , Lynne Ireland Denny , Richard R Rustandi , Geethanjali Dornadula , Brock Fiorito , Zhi-Qiang Zhang

Réseau de co-auteurs

John W Loughney 14 collaborations

Andrew R Swartz 5 collaborations

Michael A Winters 5 collaborations

Sha Ha 4 collaborations

Yvonne Shieh 4 collaborations

Melissa Hamm 3 collaborations

James Z Deng 3 collaborations

Josef Vlasak 3 collaborations

Mohamed Dawod 3 collaborations

Jessica Raffaele 3 collaborations

Adam T Sutton 3 collaborations

Kevin Minsker 3 collaborations

Anne Payne 3 collaborations

Liang Shang 3 collaborations

Kaylin A Adipietro 3 collaborations

Raquel Godoy-Ruiz 3 collaborations

Kristen M Varney 3 collaborations

Edwin Pozharski 3 collaborations

David J Weber 3 collaborations

Shiyi Wang 3 collaborations

Tian Lu 2 collaborations

Alyssa Deiss 2 collaborations

Amanda Gulasarian 2 collaborations

Ashley Prout 2 collaborations

Spyridon Konstantinidis 2 collaborations

Murphy R Poplyk 2 collaborations

Rachel Thompson 2 collaborations

Sheng-Ching Wang 2 collaborations

Laura E Kuczynski 2 collaborations

James Osborn 2 collaborations

Timothy D Culp 2 collaborations

Xiwei Zheng 2 collaborations

Dinendra L Abeyawardhane 2 collaborations

Amedee des Georges 2 collaborations

Carl Hofmann 2 collaborations

Nisarg Patel 2 collaborations

Adam Kristopeit 2 collaborations

Sianny Christanti 2 collaborations

Jessica W Olson 2 collaborations

Geethanjali Dornadula 2 collaborations

Paul F Gillespie 2 collaborations

Caleb Kinsey 1 collaboration

Kim Vuolo 1 collaboration

Lee Klein 1 collaboration

Damon Barbacci 1 collaboration

Christopher Tubbs 1 collaboration

Philip McKenna 1 collaboration

Seth R Reinhart 1 collaboration

Christine Castagna 1 collaboration

Kristen L Flor 1 collaboration

Jillian Acevedo-Skrip 1 collaboration

Christopher J Wang 1 collaboration

James R Shallow 1 collaboration

Matthew P Watson 1 collaboration

Michael L Homsy 1 collaboration

Thomas Svab 1 collaboration

Ashley Gruber 1 collaboration

Jianfang Hu 1 collaboration

Xiaohua Ye 1 collaboration

Xun Gui 1 collaboration

Daniel C Freed 1 collaboration

Zhiqiang Ku 1 collaboration

Leike Li 1 collaboration

Yuanzhi Chen 1 collaboration

Wei Xiong 1 collaboration

Xuejun Fan 1 collaboration

Hang Su 1 collaboration

Xi He 1 collaboration

Ningning Ma 1 collaboration

Amy S Espeseth 1 collaboration

Jian Liu 1 collaboration

Hua Zhu 1 collaboration

Dai Wang 1 collaboration

Ningyan Zhang 1 collaboration

Tong-Ming Fu 1 collaboration

Zhiqiang An 1 collaboration

Lee J Klein 1 collaboration

Lily Farmerie 1 collaboration

Kimberly Vuolo 1 collaboration

Megan A Mackey 1 collaboration

Geoffri Ricci 1 collaboration

Austin Kapish 1 collaboration

Joseph Davide 1 collaboration

Joseph P Califano 1 collaboration

Darrell Sehlin 1 collaboration

Lawrence W Dick 1 collaboration

Andreas Baudy 1 collaboration

Edward Bell 1 collaboration

Malini Mukherjee 1 collaboration

Shruthi Murali 1 collaboration

Spiridon E Sevdalis 1 collaboration

Izza F Nawaz 1 collaboration

Alejandro X Spittel 1 collaboration

Vitalii I Silin 1 collaboration

Malorie Fink 1 collaboration

Erin Marie Cannon 1 collaboration

Cindy Pauley 1 collaboration

Matthew Troutman 1 collaboration

Mary Shank-Retzlaff 1 collaboration

John Loughney 1 collaboration

Thorsten Verch 1 collaboration

Xingjian Xu 1 collaboration

Christopher Peralta 1 collaboration

Danya Ben-Hail 1 collaboration

Mary E Cook 1 collaboration

Braden M Roth 1 collaboration

Paul T Wilder 1 collaboration

Thomas Cleveland 1 collaboration

Alexander Grishaev 1 collaboration

Heather M Neu 1 collaboration

Sarah L J Michel 1 collaboration

Wenbo Yu 1 collaboration

Dorothy Beckett 1 collaboration

Catherine Lancaster 1 collaboration

Alexander D MacKerell 1 collaboration

Cindy J Pauley 1 collaboration

Andrew Swartz 1 collaboration

Jack B Baker 1 collaboration

William J Smith 1 collaboration

Patrick L Ahl 1 collaboration

Bei Wang 1 collaboration

Jeffrey T Blue 1 collaboration

Qiuwei Xu 1 collaboration

Matthew Onimus 1 collaboration

Yue Yuan 1 collaboration

Carrie L Anderson 1 collaboration

Chengli Zong 1 collaboration

Rong-Sheng Yang 1 collaboration

Danielle V Miller 1 collaboration

Becca Pagels 1 collaboration

Jonathan Welch 1 collaboration

Mark Haverick 1 collaboration

Xin Tong 1 collaboration

Katrina Feller 1 collaboration

James Devlin 1 collaboration

David Boyd 1 collaboration

Jon Shanter 1 collaboration

Ying Wang 1 collaboration

Li Li 1 collaboration

Qiang Tu 1 collaboration

Kaixi Zhao 1 collaboration

Emily Wen 1 collaboration

Andrew Singh 1 collaboration

Matthew J Gunsch 1 collaboration

Richard Rianto Rustandi 1 collaboration

David Foley 1 collaboration

Yu He 1 collaboration

Rijul Bhimwal 1 collaboration

Nicholas Cunningham 1 collaboration

Jessica Olson 1 collaboration

Yanjie Wang 1 collaboration

Jennifer L Teyral 1 collaboration

Christopher M Tubbs 1 collaboration

Kelsey Shiflett 1 collaboration

Cara Pearson 1 collaboration

Yi Wang 1 collaboration

Eman Alkurdi 1 collaboration

Young Zhang 1 collaboration

Kuo Yin 1 collaboration

Hualin Helen Li 1 collaboration

Jenny Xu 1 collaboration

Li He 1 collaboration

Lynne Ireland Denny 1 collaboration

Brock Fiorito 1 collaboration

Zhi-Qiang Zhang 1 collaboration