Synergistic evaluation of Moringa oleifera extract and ß-lactam antibiotic to restore the susceptibility of methicillin-resistant Staphylococcus aureus.
Anti-Bacterial Agents
/ chemistry
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Drug Synergism
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Microbial Sensitivity Tests
Moringa oleifera
/ chemistry
Phytochemicals
/ chemistry
Plant Extracts
/ chemistry
beta-Lactams
/ chemistry
Fractional inhibitory concentration
MRSA
Moringa Oleifera
PBP2a protein
Synergistic effect
β-lactamase
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
30
07
2021
accepted:
27
10
2021
pubmed:
23
11
2021
medline:
29
3
2022
entrez:
22
11
2021
Statut:
ppublish
Résumé
The antibiotic resistance has become a major threat to global health. The combinatorial use of two or more compounds to develop a new formulation may overcome the emerging cases of drug resistance. Moringa oleifera has been utilized as a strong nutritional, immunomodulator and therapeutic agent for decades. In this study, different parts of Moringa oleifera were screened for bioactive compounds that can act as a resistance modifying agent for multi-drug resistant organisms (MDROs). Initially, the combined effect of stem bark extracts and ampicillin was calculated by checkerboard assay. Active compounds of effective extract were assessed by High Performance Liquid Chromatography (HPLC). Minimal Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration Index (FICI) were calculated to evaluate the synergistic behavior of stem bark extract with ampicillin. To study the blocking of resistance pathways of Methicillin-Resistant Staphylococcus aureus (MRSA) western blot was performed. The results revealed that stem bark has significant anti-MRSA activity. The methanolic extract of stem bark in combination with ampicillin showed the highest synergistic effect (FICI value ≤ 0.237) against MRSA. Killing kinetics and membrane potential of ampicillin alone and in combination revealed an increase in the inhibitory potential of ampicillin against MRSA. Decolourization in iodometric assay confirmed the inhibition of β-lactamase, western blot results confirmed the blocking of penicillin-binding protein (PBP2a) expression with the restoration of MRSA sensitivity against β-lactams. It can be concluded that methanolic extract of Moringa oleifera stem bark has bioactive compounds and can be used as an adjuvant with antibiotics to modify the resistance of MDROs.
Identifiants
pubmed: 34807379
doi: 10.1007/s11033-021-06889-7
pii: 10.1007/s11033-021-06889-7
doi:
Substances chimiques
Anti-Bacterial Agents
0
Phytochemicals
0
Plant Extracts
0
beta-Lactams
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
421-432Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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