Analysis on RNA Motif-Based RNA Trafficking in Plants.
DIG Northern blot
RNA systemic trafficking
Site-directed mutagenesis
Viroid
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
30
11
2021
pubmed:
1
12
2021
medline:
15
1
2022
Statut:
ppublish
Résumé
Systemic RNA trafficking widely exists in plants and is critical for integrating the healthy development and responses to environmental cues. Viroids, single-stranded circular noncoding RNAs that infect plants, have been used as a model to delineate the mechanism underlying systemic RNA trafficking. Recent work on viroids has shown that structural motifs are critical to direct RNA trafficking through distinct cellular boundaries. Here, we describe the methods for generating mutational variants using site-directed mutagenesis and infection assays to unravel the function of RNA motifs. This approach can be modified to study other RNA motif-based biological processes.
Identifiants
pubmed: 34845682
doi: 10.1007/978-1-0716-1464-8_3
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
29-37Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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