A Bioluminescence Resonance Energy Transfer-Based Reporter System: Characterization and Applications.

CRISPR-Cas Systems / genetics DNA Breaks, Double-Stranded Energy Transfer Gene Editing / methods RNA, Guide, Kinetoplastida / genetics

Journal

The CRISPR journal
ISSN: 2573-1602
Titre abrégé: CRISPR J
Pays: United States
ID NLM: 101738191

Informations de publication

Date de publication:
12 2021
Historique:
pubmed: 2 12 2021
medline: 5 4 2022
entrez: 1 12 2021
Statut: ppublish

Résumé

Genome editing strategies and DNA repair research need powerful analytical tools. We generated a bioluminescence resonance energy transfer (BRET)-based reporter for the quantification of indel frequencies induced by DNA repair. The BRET reporter, expressed as a single molecule, consists of a mutated

Identifiants

DOI: 10.1089/crispr.2021.0023 PMID: 34847743
pubmed: 34847743
doi: 10.1089/crispr.2021.0023
doi:

Substances chimiques

RNA, Guide 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

884-895

Commentaires et corrections

Type : ErratumIn

Auteurs

Tobias Wimmer (T)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

Dileep Bonthu (D)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

Vincent Moeschl (V)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

Philip Kleekamp (P)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

Christian Thiel (C)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

Lyubomyr Lytvynchuk (L)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

Matthew Ellinwood (M)

National MPS Society, Durham, North Carolina, USA.

Knut Stieger (K)

Department of Ophthalmology, Justus-Liebig-University Giessen, Giessen, Germany.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › A Bioluminescence Resonance Energy...
questionsmedicales.fr › Phénomènes génétiques › Altération de l'ADN › Cassures de l'ADN › Cassures double-brin de l'ADN › A Bioluminescence Resonance Energy...
questionsmedicales.fr › Phénomènes chimiques › Transfert d'énergie › A Bioluminescence Resonance Energy...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › A Bioluminescence Resonance Energy...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › ARN guide › A Bioluminescence Resonance Energy...