Structural and Functional Analysis of SsaV Cytoplasmic Domain and Variable Linker States in the Context of the InvA-SsaV Chimeric Protein.
Bacterial Proteins
/ genetics
Cryoelectron Microscopy
Genomic Islands
/ genetics
Membrane Proteins
/ genetics
Protein Domains
/ physiology
Recombinant Fusion Proteins
/ genetics
Salmonella typhimurium
/ pathogenicity
Type III Secretion Systems
/ physiology
Virulence
/ genetics
Virulence Factors
/ genetics
Salmonella
cryo-EM
export gate protein
injectisome
type III secretion system
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
22 12 2021
22 12 2021
Historique:
pubmed:
2
12
2021
medline:
24
2
2022
entrez:
1
12
2021
Statut:
ppublish
Résumé
The type III secretion (T3S) injectisome is a syringe-like protein-delivery nanomachine widely utilized by Gram-negative bacteria. It can deliver effector proteins directly from bacteria into eukaryotic host cells, which is crucial for the bacterial-host interaction. Intracellular pathogen Salmonella enterica serovar Typhimurium encodes two sets of T3S injectisomes from Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2), which are critical for its host invasion and intracellular survival, respectively. The inner membrane export gate protein, SctV (InvA in SPI-1 and SsaV in SPI-2), is the largest component of the injectisome and is essential for assembly and function of T3SS. Here, we report the 2.11 Å cryo-EM structure of the SsaV cytoplasmic domain (SsaV
Identifiants
pubmed: 34851139
doi: 10.1128/Spectrum.01251-21
pmc: PMC8635156
doi:
Substances chimiques
Bacterial Proteins
0
Membrane Proteins
0
Recombinant Fusion Proteins
0
Type III Secretion Systems
0
Virulence Factors
0
invA protein, Bacteria
147652-43-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0125121Références
Mol Syst Biol. 2011 Oct 11;7:539
pubmed: 21988835
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24786-24795
pubmed: 31744874
J Struct Biol. 2012 Dec;180(3):519-30
pubmed: 23000701
FEMS Microbiol Lett. 2018 Jun 1;365(12):
pubmed: 29850796
Sci Adv. 2018 Apr 25;4(4):eaao7054
pubmed: 29707633
Proc Natl Acad Sci U S A. 2015 Jan 27;112(4):1047-52
pubmed: 25583506
EMBO J. 2005 Jun 1;24(11):2043-52
pubmed: 15889142
Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17745-50
pubmed: 20876096
J Mol Graph. 1996 Dec;14(6):354-60, 376
pubmed: 9195488
Nat Struct Mol Biol. 2014 Jan;21(1):82-7
pubmed: 24317488
J Struct Biol. 2020 Dec 1;212(3):107660
pubmed: 33129970
PLoS Pathog. 2016 Mar 04;12(3):e1005495
pubmed: 26943926
Nat Protoc. 2015 Jun;10(6):845-58
pubmed: 25950237
Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):485-90
pubmed: 17202259
Structure. 2019 Jun 4;27(6):965-976.e6
pubmed: 31031200
Microbiologyopen. 2018 Jun;7(3):e00571
pubmed: 29277965
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Cell Microbiol. 2003 Aug;5(8):501-11
pubmed: 12864810
Infect Immun. 1995 Feb;63(2):729-32
pubmed: 7822051
J Struct Biol. 2016 Jan;193(1):1-12
pubmed: 26592709
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
Science. 2010 May 21;328(5981):1040-3
pubmed: 20395475
Nat Commun. 2011 Sep 20;2:475
pubmed: 21934659
Cell. 2017 Mar 9;168(6):1065-1074.e10
pubmed: 28283062
Nat Commun. 2015 Dec 11;6:10114
pubmed: 26656452
Science. 2011 Mar 4;331(6021):1192-5
pubmed: 21385715
Mol Microbiol. 2010 Apr;76(1):260-8
pubmed: 20199603
Cell Microbiol. 2018 Sep;20(9):e12939
pubmed: 30030897
Biomolecules. 2019 Jul 14;9(7):
pubmed: 31337100
Elife. 2019 Apr 01;8:
pubmed: 30932812
Science. 2019 Jun 21;364(6446):
pubmed: 31221832
Curr Microbiol. 2013 Jun;66(6):582-7
pubmed: 23370732
J Bacteriol. 1992 Jul;174(13):4338-49
pubmed: 1624429
Microbiology (Reading). 2013 Mar;159(Pt 3):446-461
pubmed: 23288540
Science. 2004 Nov 5;306(5698):1040-2
pubmed: 15528446
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W299-302
pubmed: 15980475
mBio. 2014 Feb 04;5(1):e00946-13
pubmed: 24496791
Annu Rev Microbiol. 2014;68:415-38
pubmed: 25002086
Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):6098-6103
pubmed: 28533372
Science. 2011 Mar 4;331(6021):1188-91
pubmed: 21292939
Curr Opin Struct Biol. 2020 Apr;61:71-78
pubmed: 31821956
Nature. 2006 Nov 30;444(7119):567-73
pubmed: 17136086
Nat Rev Drug Discov. 2010 Feb;9(2):117-28
pubmed: 20081869
J Mol Biol. 2021 Oct 15;433(21):167188
pubmed: 34454944
Nat Microbiol. 2021 Jun;6(6):712-721
pubmed: 33931760
Nat Struct Mol Biol. 2013 Jan;20(1):99-104
pubmed: 23222644
Nat Commun. 2018 May 2;9(1):1773
pubmed: 29720631
Mol Microbiol. 2004 Nov;54(3):604-19
pubmed: 15491354
Annu Rev Microbiol. 2020 Sep 8;74:181-200
pubmed: 32603624
Nat Struct Mol Biol. 2011 Mar;18(3):277-82
pubmed: 21278755
Protein Sci. 2010 May;19(5):1091-6
pubmed: 20306492
Nat Commun. 2018 Sep 21;9(1):3840
pubmed: 30242280
PLoS One. 2016 May 12;11(5):e0155141
pubmed: 27171191
Cell. 2021 May 13;184(10):2665-2679.e19
pubmed: 33882274
Mol Microbiol. 2002 Mar;43(5):1089-103
pubmed: 11918798
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
PLoS One. 2021 Jun 18;16(6):e0252800
pubmed: 34143799
Nat Rev Microbiol. 2017 Jun;15(6):323-337
pubmed: 28392566
Nat Commun. 2019 Feb 7;10(1):626
pubmed: 30733444
Curr Top Microbiol Immunol. 2018;416:73-115
pubmed: 30178263
Trends Microbiol. 2015 May;23(5):296-300
pubmed: 25701111
PLoS Pathog. 2020 Oct 13;16(10):e1008923
pubmed: 33048983
Front Cell Infect Microbiol. 2019 Jul 31;9:270
pubmed: 31428589
PLoS Pathog. 2014 Nov 06;10(11):e1004498
pubmed: 25375170
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
Cell Host Microbe. 2017 Aug 9;22(2):217-231
pubmed: 28799907
Mol Microbiol. 2017 Apr;104(2):234-249
pubmed: 28106310
J Struct Biol. 2005 Apr;150(1):69-80
pubmed: 15797731
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679):
pubmed: 26370933
Cold Spring Harb Perspect Biol. 2010 Nov;2(11):a000299
pubmed: 20926516
J Struct Biol. 2021 Jun;213(2):107729
pubmed: 33774138
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Biochim Biophys Acta. 2014 Aug;1843(8):1649-63
pubmed: 24512838