DNA repair inhibitors sensitize cells differently to high and low LET radiation.
Alpha Particles
Ataxia Telangiectasia Mutated Proteins
/ pharmacology
Cell Cycle
/ drug effects
Cell Cycle Checkpoints
/ drug effects
Cell Line, Tumor
Cell Survival
/ drug effects
DNA Breaks, Double-Stranded
/ drug effects
DNA Repair
/ drug effects
DNA-Activated Protein Kinase
/ pharmacology
HEK293 Cells
Histones
/ metabolism
Humans
Linear Energy Transfer
Micronucleus Tests
Radiation Tolerance
/ drug effects
Radiation, Ionizing
Radiation-Sensitizing Agents
/ pharmacology
Radiometry
X-Rays
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
received:
15
12
2020
accepted:
15
11
2021
entrez:
2
12
2021
pubmed:
3
12
2021
medline:
27
1
2022
Statut:
epublish
Résumé
The aim of this study was to investigate effects of high LET α-radiation in combination with inhibitors of DDR (DNA-PK and ATM) and to compare the effect with the radiosensitizing effect of low LET X-ray radiation. The various cell lines were irradiated with α-radiation and with X-ray. Clonogenic survival, the formation of micronuclei and cell cycle distribution were studied after combining of radiation with DDR inhibitors. The inhibitors sensitized different cancer cell lines to radiation. DNA-PKi affected survival rates in combination with α-radiation in selected cell lines. The sensitization enhancement ratios were in the range of 1.6-1.85 in cancer cells. ATMi sensitized H460 cells and significantly increased the micronucleus frequency for both radiation qualities. ATMi in combination with α-radiation reduced survival of HEK293. A significantly elicited cell cycle arrest in G
Identifiants
pubmed: 34853427
doi: 10.1038/s41598-021-02719-9
pii: 10.1038/s41598-021-02719-9
pmc: PMC8636489
doi:
Substances chimiques
Histones
0
Radiation-Sensitizing Agents
0
ATM protein, human
EC 2.7.11.1
Ataxia Telangiectasia Mutated Proteins
EC 2.7.11.1
DNA-Activated Protein Kinase
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23257Informations de copyright
© 2021. The Author(s).
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