Neutron encoded derivatization of endothelial cell lysates for quantitation of aldehyde metabolites using nESI-LC-HRMS.
Aldehyde
Derivatization
Multiplex
NeuCode
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
15 Jan 2022
15 Jan 2022
Historique:
received:
13
09
2021
revised:
27
10
2021
accepted:
06
11
2021
entrez:
3
12
2021
pubmed:
4
12
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Biological aldehydes are difficult to analyze by electrospray ionization mass spectrometry due to their poor proton affinity and low biological concentrations. Chemical derivatization with stable isotope tags is used here for sample multiplexing, increased throughput, improved signal intensity, and quantitation. Nine quaternary amine tags with mass differences as low as 0.0058 Da had no observable chromatographic shifts, small amounts of ion suppression, and minimal matrix effects. Low concentration perfluoropentanoic acid was used as an ion pairing reagent to improve the retention of derivatized aldehydes. Perfluoropentanoic acid addition showed an average of three-fold improvement in limits of detection, 50% reduction in peak width, and 2.5 fold increase in analyte retention. Analysis of fifteen tagged aldehydes yielded an average of 13 nM limit of detection, 9 %RSD, R
Identifiants
pubmed: 34857138
pii: S0003-2670(21)01086-2
doi: 10.1016/j.aca.2021.339260
pmc: PMC8646956
mid: NIHMS1756749
pii:
doi:
Substances chimiques
Aldehydes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339260Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM134081
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
J Am Soc Mass Spectrom. 2020 Sep 2;31(9):1854-1860
pubmed: 32678615
Anal Chem. 2020 Jan 7;92(1):67-84
pubmed: 31639301
Mol Cell Proteomics. 2014 Sep;13(9):2503-12
pubmed: 24938287
Anal Chem. 2015 Aug 4;87(15):7660-6
pubmed: 26114225
Free Radic Biol Med. 2017 Oct;111:294-308
pubmed: 28192230
Anal Chem. 2014 Sep 16;86(18):9065-73
pubmed: 25137547
J Am Soc Mass Spectrom. 2020 Sep 2;31(9):1965-1973
pubmed: 32840365
Endocrinology. 2012 May;153(5):2099-111
pubmed: 22396448
Mol Cell Proteomics. 2013 Nov;12(11):3360-9
pubmed: 23882030
Oxid Med Cell Longev. 2014;2014:360438
pubmed: 24999379
Mass Spectrom Rev. 2020 Nov 25;:
pubmed: 33238061
Anal Chem. 2018 Nov 20;90(22):13514-13522
pubmed: 30339755
Anal Chem. 2014 May 6;86(9):4402-8
pubmed: 24684282
Diabetes. 2014 Jan;63(1):89-100
pubmed: 24062247
Anal Chem. 2013 Sep 3;85(17):8188-95
pubmed: 23895018
Nat Methods. 2019 Nov;16(11):1123-1130
pubmed: 31611692
Anal Chem. 2017 Jun 20;89(12):6758-6765
pubmed: 28505421
Anal Chem. 2019 Sep 17;91(18):11794-11802
pubmed: 31356052
J Proteome Res. 2011 Nov 4;10(11):5242-50
pubmed: 21961526
J Chromatogr A. 2013 Jul 26;1300:199-203
pubmed: 23602639
Anal Chem. 2020 Jul 7;92(13):9305-9311
pubmed: 32466643
Anal Chem. 2007 Jul 15;79(14):5143-9
pubmed: 17563114
Analyst. 2015 Aug 7;140(15):5276-86
pubmed: 26086784
Electrophoresis. 2020 Aug;41(15):1374-1378
pubmed: 32548848
Talanta. 2014 Mar;120:84-93
pubmed: 24468346
J Mass Spectrom. 2007 Feb;42(2):187-98
pubmed: 17154347
Chem Commun (Camb). 2013 Dec 7;49(94):11080-2
pubmed: 24141386
Anal Chem. 2012 Sep 4;84(17):7469-78
pubmed: 22880955
Anal Chem. 2013 Jun 4;85(11):5288-96
pubmed: 23590404
Nat Protoc. 2008;3(8):1299-311
pubmed: 18714298
Anal Chem. 2020 Jun 16;92(12):8228-8234
pubmed: 32401496
J Proteome Res. 2019 Mar 1;18(3):1078-1087
pubmed: 30638020
Anal Chem. 2002 Aug 1;74(15):3662-9
pubmed: 12175151
Anal Chem. 2008 Dec 1;80(23):9042-51
pubmed: 19551932
Free Radic Biol Med. 2005 Jun 1;38(11):1526-36
pubmed: 15890627
Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Jul;1865(7):158681
pubmed: 32171908
Anal Chim Acta. 2017 Oct 9;989:59-70
pubmed: 28915943
Rapid Commun Mass Spectrom. 2003;17(24):2815-21
pubmed: 14673832
Anal Chem. 2019 Jan 2;91(1):178-189
pubmed: 30525468
Nat Methods. 2013 Apr;10(4):332-4
pubmed: 23435260
J Chromatogr A. 2007 Nov 23;1172(2):127-34
pubmed: 17961581
Anal Chem. 2021 May 4;93(17):6629-6637
pubmed: 33880916
Anal Bioanal Chem. 2019 Sep;411(24):6399-6407
pubmed: 31372700
J Am Chem Soc. 2003 Nov 12;125(45):13836-49
pubmed: 14599224
J Am Soc Mass Spectrom. 2019 Sep;30(9):1779-1789
pubmed: 31250320
Chem Commun (Camb). 2021 Jan 26;57(6):737-740
pubmed: 33496701
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Apr 15;988:149-56
pubmed: 25777478
Anal Chem. 2018 Dec 4;90(23):13867-13875
pubmed: 30408416
Oxid Med Cell Longev. 2013;2013:629028
pubmed: 23819013