Accelerating strain phenotyping with desorption electrospray ionization-imaging mass spectrometry and untargeted analysis of intact microbial colonies.
DESI-IMS
free fatty acid profiling
imaging mass spectrometry
multiplexed metabolomics
synthetic biology
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
07 12 2021
07 12 2021
Historique:
accepted:
26
10
2021
entrez:
3
12
2021
pubmed:
4
12
2021
medline:
19
1
2022
Statut:
ppublish
Résumé
Reading and writing DNA were once the rate-limiting step in synthetic biology workflows. This has been replaced by the search for the optimal target sequences to produce systems with desired properties. Directed evolution and screening mutant libraries are proven technologies for isolating strains with enhanced performance whenever specialized assays are available for rapidly detecting a phenotype of interest. Armed with technologies such as CRISPR-Cas9, these experiments are capable of generating libraries of up to 10
Identifiants
pubmed: 34857637
pii: 2109633118
doi: 10.1073/pnas.2109633118
pmc: PMC8670462
pii:
doi:
Substances chimiques
Fatty Acids
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R03 CA222452
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM107434
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067152
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM092218
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM067152
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM128344
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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